ArticleTropical animal health and production2018
Development and evaluation of a nested-PCR assay for Senecavirus A diagnosis.
Article in Tropical animal health and production, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of Senecavirus A in pigs from 2014 to 2020: a global systematic review and meta-analysis.Journal of veterinary science · 2023Pooled it
- Serological and molecular evaluation of Senecavirus A (SVA) in pigs from farrow-to-finish farms in Minas Gerais, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Article
- Rapid Visual Detection of Senecavirus A Based on RPA-CRISPR/Cas12a System with Canonical or Suboptimal PAM.Viruses · 2025Article
- Isolation and Characterization of Seneca Valley Virus From Pig Transboundary Spread to the Mink Infection.Transboundary and emerging diseases · 2025Article
- Rapid molecular detection ofFrontiers in bioengineering and biotechnology · 2025Article
- Development of a CRISPR/Cas12a-based fluorescent detection method of Senecavirus A.BMC veterinary research · 2024Article
- Real-time detection of Seneca Valley virus by one-tube RPA-CRISPR/Cas12a assay.Frontiers in cellular and infection microbiology · 2023Article
- A Review on Pathological and Diagnostic Aspects of Emerging Viruses-Veterinary sciences · 2022Review
- Detection methods targeting the positive- and negative-sense RNA transcripts from plus-stranded RNA viruses.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2022Article
- Advances in the differential molecular diagnosis of vesicular disease pathogens in swine.Frontiers in microbiology · 2022Review
- Porcine rotavirus B as primary causative agent of diarrhea outbreaks in newborn piglets.Scientific reports · 2020Article
- Characterization of Emerging Swine Viral Diseases through Oxford Nanopore Sequencing Using Senecavirus A as a Model.Viruses · 2020Article
- A 5-Year Review of Senecavirus A in China since Its Emergence in 2015.Frontiers in veterinary science · 2020Review
- Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus.BMC veterinary research · 2019Article
- Rapid detection of low-level HeLa cell contamination in cell culture using nested PCR.Journal of cellular and molecular medicine · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Senecavirus A (SVA) has been associated with vesicular disease in weaned and adult pigs and with high mortality of newborn piglets. This study aimed to establish a nested-PCR assay for the routine diagnosis of SVA infection. Tissue samples (n = 177) were collected from 37 piglets of 18 pig farms located in four different Brazilian states. For the nested-PCR, a primer set was defined to amplify an internal VP1 fragment of 316 bp of SVA genome. Of the 37 piglets, 15 (40.5%) and 23 (62.2%) were positive for the SVA in the RT-PCR and nested-PCR assays, respectively. The SVA RNA was detected in 61/177 (34.5%) samples with the RT-PCR, while the nested-PCR assay showed 84/177 (47.5%) samples with the virus (p < 0.05). According to the herds, 11 (61.1%) and 16 (88.9%) of the 18 pig herds were positive for the SVA in the RT-PCR and nested-PCR assays, respectively. Nucleotide sequencing analysis revealed similarities of 98.7-100% among SVA Brazilian strains and of 86.6-98% with SVA strains from other countries. The nested-PCR assay in this study was suitable to recover the SVA RNA in biological specimens, piglets, and/or herds that were considered as negative in the RT-PCR assay, and is proposed for the routine investigation of the SVA infection in piglets, especially when other techniques are not available or when a great number of samples has to be examined.
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