ArticleMethods (San Diego, Calif.)2018
Optimized processing and analysis of conventional confocal microscopy generated scanning FCS data.
Article in Methods (San Diego, Calif.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Spatial triple-correlation spectroscopy reveals heterotrimer dynamics in live cells.Biophysical journal · 2026Article
- Article
- HCR-Proxy resolves site-specific proximal RNA microenvironments at subcompartmental resolution.Nucleic acids research · 2026Article
- Quantification of membrane fluidity in bacteria using TIR-FCS.Biophysical journal · 2024Article
- Quantifying biomolecular organisation in membranes with brightness-transit statistics.Nature communications · 2024Article
- Neural network informed photon filtering reduces fluorescence correlation spectroscopy artifacts.Biophysical journal · 2024Article
- Transmembrane domain-driven PD-1 dimers mediate T cell inhibition.Science immunology · 2024Article
- Using CombiCells, a platform for titration and combinatorial display of cell surface ligands, to study T-cell antigen sensitivity modulation by accessory receptors.The EMBO journal · 2024Article
- The kinase occupancy of T cell coreceptors reconsidered.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Using FCS to accurately measure protein concentration in the presence of noise and photobleaching.Biophysical journal · 2021Article
- Article
- Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins.iScience · 2021Article
- High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy.Journal of physics D: Applied physics · 2020Article
- The cortical actin network regulates avidity-dependent binding of hyaluronan by the lymphatic vessel endothelial receptor LYVE-1.The Journal of biological chemistry · 2020Article
- Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells.Frontiers in immunology · 2020Review
- HIV-1 Gag specifically restricts PI(4,5)P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly.Science advances · 2019Article
- Nanoscale dynamics of cholesterol in the cell membrane.The Journal of biological chemistry · 2019Article
- Adaptive optics allows STED-FCS measurements in the cytoplasm of living cells.Optics express · 2019Article
- Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED-FCS.Nature protocols · 2019Article
- Statistical Analysis of Scanning Fluorescence Correlation Spectroscopy Data Differentiates Free from Hindered Diffusion.ACS nano · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Scanning Fluorescence Correlation Spectroscopy (scanning FCS) is a variant of conventional point FCS that allows molecular diffusion at multiple locations to be measured simultaneously. It enables disclosure of potential spatial heterogeneity in molecular diffusion dynamics and also the acquisition of a large amount of FCS data at the same time, providing large statistical accuracy. Here, we optimize the processing and analysis of these large-scale acquired sets of FCS data. On one hand we present FoCuS-scan, scanning FCS software that provides an end-to-end solution for processing and analysing scanning data acquired on commercial turnkey confocal systems. On the other hand, we provide a thorough characterisation of large-scale scanning FCS data over its intended time-scales and applications and propose a unique solution for the bias and variance observed when studying slowly diffusing species. Our manuscript enables researchers to straightforwardly utilise scanning FCS as a powerful technique for measuring diffusion across a broad range of physiologically relevant length scales without specialised hardware or expensive software.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.