Evidence map›Paper›PMID 28962663›Full record

ArticleStem cell research & therapy2017

Lineage- and developmental stage-specific mechanomodulation of induced pluripotent stem cell differentiation.

Maricela Maldonado, Rebeccah J Luu, Gerardo Ico, Alex Ospina, Danielle Myung, Hung Ping Shih, Jin Nam

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Using 2D and 3D pluripotent stem cell models to study neurotropic viruses.Frontiers in virology (Lausanne, Switzerland) · 2022
    Article
  8. Review
  9. Review
  10. Article
  11. Maneuvering the Migration and Differentiation of Stem Cells with Electrospun Nanofibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020
    Review
  12. Review
  13. Micro-Engineered Models of Development Using Induced Pluripotent Stem Cells.Frontiers in bioengineering and biotechnology · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maricela MaldonadoDepartment of Bioengineering, University of California-Riverside, Materials Science & Engineering Building 331, 900 University Avenue, Riverside, CA, 92521, USA.
Rebeccah J LuuDepartment of Bioengineering, University of California-Riverside, Materials Science & Engineering Building 331, 900 University Avenue, Riverside, CA, 92521, USA.
Gerardo IcoDepartment of Bioengineering, University of California-Riverside, Materials Science & Engineering Building 331, 900 University Avenue, Riverside, CA, 92521, USA.
Alex OspinaDepartment of Bioengineering, University of California-Riverside, Materials Science & Engineering Building 331, 900 University Avenue, Riverside, CA, 92521, USA.
Danielle MyungDepartment of Bioengineering, University of California-Riverside, Materials Science & Engineering Building 331, 900 University Avenue, Riverside, CA, 92521, USA.
Hung Ping ShihDepartment of Translational Research and Cellular Therapeutics, City of Hope, Duarte, California, 91010, USA.
Jin NamDepartment of Bioengineering, University of California-Riverside, Materials Science & Engineering Building 331, 900 University Avenue, Riverside, CA, 92521, USA. jnam@engr.ucr.edu.ORCID 0000-0001-5117-8958

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTo maximize the translational utility of human induced pluripotent stem cells (iPSCs), the ability to precisely modulate the differentiation of iPSCs to target phenotypes is critical. Although the effects of the physical cell niche on stem cell differentiation are well documented, current approaches to direct step-wise differentiation of iPSCs have been typically limited to the optimization of soluble factors. In this regard, we investigated how temporally varied substrate stiffness affects the step-wise differentiation of iPSCs towards various lineages/phenotypes.

methodsElectrospun nanofibrous substrates with different reduced Young's modulus were utilized to subject cells to different mechanical environments during the differentiation process towards representative phenotypes from each of three germ layer derivatives including motor neuron, pancreatic endoderm, and chondrocyte. Phenotype-specific markers of each lineage/stage were utilized to determine differentiation efficiency by reverse-transcription polymerase chain reaction (RT-PCR) and immunofluorescence imaging for gene and protein expression analysis, respectively.

resultsThe results presented in this proof-of-concept study are the first to systematically demonstrate the significant role of the temporally varied mechanical microenvironment on the differentiation of stem cells. Our results demonstrate that the process of differentiation from pluripotent cells to functional end-phenotypes is mechanoresponsive in a lineage- and differentiation stage-specific manner.

conclusionsLineage/developmental stage-dependent optimization of electrospun substrate stiffness provides a unique opportunity to enhance differentiation efficiency of iPSCs for their facilitated therapeutic applications.

Indexed as

Cell DifferentiationCell LineCell LineageElastic ModulusHumansInduced Pluripotent Stem CellsNanofibersPrimary Cell CultureTissue ScaffoldsDifferentiationInduced pluripotent stem cellsMechanobiologySubstrate stiffness

Identifiers

PMID28962663
PMCPMC5622562

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.