Evidence map›Paper›PMID 28962589›Full record

ArticleStem cell research & therapy2017

Multipotent mesenchymal stromal cells enhance insulin secretion from human islets via N-cadherin interaction and prolong function of transplanted encapsulated islets in mice.

Elisa Montanari, Raphael P H Meier, Redouan Mahou, Jörg D Seebach, Christine Wandrey, Sandrine Gerber-Lemaire, Leo H Buhler, Carmen Gonelle-Gispert

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.

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  11. Cotransplantation of marginal mass allogeneic islets with 3D culture-derived adult human skin cells improves glycemia in diabetic mice.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Elisa MontanariDepartment of Surgery, Geneva University Hospitals and Medical Faculty, 1211, Geneva, Switzerland.
Raphael P H MeierDepartment of Surgery, Geneva University Hospitals and Medical Faculty, 1211, Geneva, Switzerland.
Redouan MahouInstitute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
Jörg D SeebachDivision of Immunology and Allergy, Geneva University Hospitals and Medical Faculty, 1211, Geneva, Switzerland.
Christine WandreyInstitute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Sandrine Gerber-LemaireInstitute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Leo H BuhlerDepartment of Surgery, Geneva University Hospitals and Medical Faculty, 1211, Geneva, Switzerland.
Carmen Gonelle-GispertDepartment of Surgery, Geneva University Hospitals and Medical Faculty, 1211, Geneva, Switzerland. Carmen.Gonelle@unige.ch.
University Hospital of Geneva · CHÉcole Polytechnique Fédérale de Lausanne · CHUniversity of Toronto · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMultipotent mesenchymal stromal cells (MSC) enhance viability and function of islets of Langerhans. We aimed to examine the interactions between human MSC and human islets of Langerhans that influence the function of islets.

methodsHuman MSC and human islets (or pseudoislets, obtained after digestion and reaggregation of islet cells) were cocultured with or without cellular contact and glucose-stimulated insulin secretion assays were performed to assess cell function. The expression of several adhesion molecules, notably ICAM-1 and N-cadherin on islets and MSC, was investigated by qPCR. The role of N-cadherin was analyzed by adding an anti-N-cadherin antibody in islets cultured with or without MSC for 24 h followed by insulin measurements in static incubation assays. Islets and MSC were coencapsulated in new hydrogel microspheres composed of calcium alginate and covalently crosslinked polyethylene glycol. Encapsulated cells were transplanted intraperitoneally in streptozotocin-induced diabetic mice and glycemia was monitored. Islet function was evaluated by the intraperitoneal glucose tolerance test.

resultsIn vitro, free islets and pseudoislets cocultured in contact with MSC showed a significantly increased insulin secretion when compared to islets or pseudoislets cultured alone or cocultured without cell-to-cell contact with MSC (p < 0.05). The expression of ICAM-1 and N-cadherin was present on islets and MSC. Blocking N-cadherin prevented the enhanced insulin secretion by islets cultured in contact with MSC whereas it did not affect insulin secretion by islets cultured alone. Upon transplantation in diabetic mice, islets microencapsulated together with MSC showed significantly prolonged normoglycemia when compared with islets alone (median 69 and 39 days, respectively, p < 0.01). The intraperitoneal glucose tolerance test revealed an improved glycemic response in mice treated with islets microencapsulated together with MSC compared to mice transplanted with islets alone (p < 0.001).

conclusionsMSC improve survival and function of islets of Langerhans by cell-to-cell contact mediated by the adhesion molecule N-cadherin.

Indexed as

AlginatesAnimalsBlood GlucoseCadherinsCells, CulturedDiabetes Mellitus, ExperimentalGlucuronic AcidHexuronic AcidsHumansHydrogelsInsulinInsulin SecretionIslets of LangerhansIslets of Langerhans TransplantationMaleMesenchymal Stem CellsAlginatesBlood GlucoseCadherinsGlucuronic AcidHexuronic AcidsHydrogelsInsulinPolyethylene GlycolsCell interactionEncapsulationHuman isletsMesenchymal stromal cellsN-cadherin

Identifiers

PMID28962589
PMCPMC5622460
OpenAlexW2749929464

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.