ArticleMolecular biology and evolution2017
Adaptive Mutations in RNA Polymerase and the Transcriptional Terminator Rho Have Similar Effects on Escherichia coli Gene Expression.
Article in Molecular biology and evolution, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Toward a systems metabolic engineering approach to improve thermotolerance of Escherichia coli.Microbial cell factories · 2026Review
- Enhancing the heat tolerance of homolactic Escherichia coli through reverse metabolic engineering.Applied microbiology and biotechnology · 2026Article
- Thermal Plasticity and Evolutionary Constraints inBiology · 2024Article
- Patterns of Fitness and Gene Expression Epistasis Generated by Beneficial Mutations in the rho and rpoB Genes of Escherichia coli during High-Temperature Adaptation.Molecular biology and evolution · 2024Article
- Diversification of the Rho transcription termination factor in bacteria.Nucleic acids research · 2024Article
- Experimental Evolution in a Warming World: The Omics Era.Molecular biology and evolution · 2024Review
- Convergent genetic adaptation ofFrontiers in molecular biosciences · 2024Article
- Pervasive genotype-by-environment interactions shape the fitness effects of antibiotic resistance mutations.Proceedings. Biological sciences · 2023Article
- The hallmarks of a tradeoff in transcriptomes that balances stress and growth functions.Research square · 2023Article
- Article
- Laboratory evolution of synthetic electron transport system variants reveals a larger metabolic respiratory system and its plasticity.Nature communications · 2022Article
- Chemical-genetic interrogation of RNA polymerase mutants reveals structure-function relationships and physiological tradeoffs.Molecular cell · 2021Article
- Genome-wide insights into population structure and host specificity of Campylobacter jejuni.Scientific reports · 2021Article
- Mastering the control of the Rho transcription factor for biotechnological applications.Applied microbiology and biotechnology · 2021Review
- Evolutionary dynamics and structural consequences of de novo beneficial mutations and mutant lineages arising in a constant environment.BMC biology · 2021Article
- Distinct clonal lineages and within-host diversification shape invasive Staphylococcus epidermidis populations.PLoS pathogens · 2021Article
- Emerging Adaptive Strategies Under Temperature Fluctuations in a Laboratory Evolution Experiment ofFrontiers in microbiology · 2021Article
- Genetic Mutations That Drive Evolutionary Rescue to Lethal Temperature in Escherichia coli.Genome biology and evolution · 2020Article
- Escherichia coli adaptation and response to exposure to heavy atmospheric pollution.Scientific reports · 2019Article
- Review
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Modifications to transcriptional regulators play a major role in adaptation. Here, we compared the effects of multiple beneficial mutations within and between Escherichia coli rpoB, the gene encoding the RNA polymerase β subunit, and rho, which encodes a transcriptional terminator. These two genes have harbored adaptive mutations in numerous E. coli evolution experiments but particularly in our previous large-scale thermal stress experiment, where the two genes characterized alternative adaptive pathways. To compare the effects of beneficial mutations, we engineered four advantageous mutations into each of the two genes and measured their effects on fitness, growth, gene expression and transcriptional termination at 42.2 °C. Among the eight mutations, two rho mutations had no detectable effect on relative fitness, suggesting they were beneficial only in the context of epistatic interactions. The remaining six mutations had an average relative fitness benefit of ∼20%. The rpoB mutations affected the expression of ∼1,700 genes; rho mutations affected the expression of fewer genes but most (83%) were a subset of those altered by rpoB mutants. Across the eight mutants, relative fitness correlated with the degree to which a mutation restored gene expression back to the unstressed, 37.0 °C state. The beneficial mutations in the two genes did not have identical effects on fitness, growth or gene expression, but they caused parallel phenotypic effects on gene expression and genome-wide transcriptional termination.
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