ArticleCNS neuroscience & therapeutics2017
miR-181d-5p promotes neurite outgrowth in PC12 Cells via PI3K/Akt pathway.
Article in CNS neuroscience & therapeutics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Bibliometric analysis of PTEN in neurodevelopment and neurodegeneration.Frontiers in aging neuroscience · 2024Article
- Identification and Validation of Ferroptosis-Related Genes in Patients with Acute Spinal Cord Injury.Molecular neurobiology · 2023Article
- Erythropoietin inhibits ferroptosis and ameliorates neurological function after spinal cord injury.Neural regeneration research · 2023Article
- Direct targeting of DOCK4 by miRNA-181d in oxygen-glucose deprivation/reoxygenation-mediated neuronal injury.Lipids in health and disease · 2023Article
- Analysis of shared ceRNA networks and related-hub genes in rats with primary and secondary photoreceptor degeneration.Frontiers in neuroscience · 2023Article
- Ursolic Acid Ameliorates Spinal Cord Injury in Mice by Regulating Gut Microbiota and Metabolic Changes.Frontiers in cellular neuroscience · 2022Article
- Pax3 inhibits Neuro-2a cells proliferation and neurite outgrowth.Journal of cellular and molecular medicine · 2021Article
- Schwann Cell-Derived Exosomes Induce the Differentiation of Human Adipose-Derived Stem Cells Into Schwann Cells.Frontiers in molecular biosciences · 2021Article
- Inflammatory response and oxidative stress attenuated by sulfiredoxin‑1 in neuron‑like cells depends on nuclear factor erythroid‑2‑related factor 2.Molecular medicine reports · 2020Article
- The inhibition of miR-17-5p promotes cortical neuron neurite growth via STAT3/GAP-43 pathway.Molecular biology reports · 2020Article
- RhoA-GTPase Modulates Neurite Outgrowth by Regulating the Expression of Spastin and p60-Katanin.Cells · 2020Article
- miR-181d-5p promotes neurite outgrowth in PC12 Cells via PI3K/Akt pathway.CNS neuroscience & therapeutics · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionmiRNAs dysregulate in spinal cord injury (SCI) and have been demonstrated to play a crucial role in neurite outgrowth. However, the underlying mechanism remains elusive. In this study, we constructed a mouse model of SCI, extracted RNA from injured spinal cord tissue for the use of microarray assay. miR-181d-5p which is one of the most significantly expressed miRNAs in miRNA-mRNA network, abundantly expressed in center system and highly conserved across different spices, was chosen for our further study.
aimsTo demonstrate whether miR-181d-5p can promote neurite outgrowth in PC12 cells via PI3K/Akt signaling pathway, we performed function analysis of miR-181d-5p with LV-miR-181d-5p and LV-sh-GFP to infect PC12 cells.
resultsThrough microarray assay analysis, we totally found 262 significantly expressed miRNAs and 2973 target genes in SCI and observed that their expression dynamically changed postinjury. Here, we provided enough evidences that the overexpression of miR181d-5p significantly decreased the expression of PTEN, upregulated p-Akt expression, increased neurite outgrowth-related proteins (GAP-43 and NF-200) and synaptic vesicle-related proteins (Synapsin and PSD95), and then promoted neurite outgrowth in PC12 cells. Furthermore, we confirmed that miR-181d-5p could directly target to the 3'-UTR of PTEN mRNA through dual-luciferase report assay.
conclusionsOur study supports that aberrant expression of miRNAs is involved in the pathogenesis of SCI, miR-181d-5p plays an important role in neurite growth in PC12 cells via PI3K/Akt signaling pathway and may be a candidate target for the treatment of SCI in the future.
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