Evidence map›Paper›PMID 28960885›Full record

ArticleCancer medicine2017

Expression profiling of long noncoding RNA identifies lnc-MMP3-1 as a prognostic biomarker in external auditory canal squamous cell carcinoma.

Hong Liu, Chunfu Dai, Qianru Wu, Hongyan Liu, Feitian Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Article
  6. Observational
  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Hong LiuDepartment of Otology and Skull Base Surgery, Hearing Research Key Lab of Health Ministry of China, Eye & Ear Nose and Throat Hospital, Fudan University, Shanghai, China.ORCID http://orcid.org/0000-0002-1447-1300
Chunfu DaiDepartment of Otology and Skull Base Surgery, Hearing Research Key Lab of Health Ministry of China, Eye & Ear Nose and Throat Hospital, Fudan University, Shanghai, China.
Qianru WuDepartment of Otology and Skull Base Surgery, Hearing Research Key Lab of Health Ministry of China, Eye & Ear Nose and Throat Hospital, Fudan University, Shanghai, China.
Hongyan LiuDepartment of Otology and Skull Base Surgery, Hearing Research Key Lab of Health Ministry of China, Eye & Ear Nose and Throat Hospital, Fudan University, Shanghai, China.
Feitian LiDepartment of Otology and Skull Base Surgery, Hearing Research Key Lab of Health Ministry of China, Eye & Ear Nose and Throat Hospital, Fudan University, Shanghai, China.
Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Our previous studies suggested external auditory canal squamous cell carcinoma (EACSCC) is a rare malignancy with heterogeneous outcomes. This study aimed to identify lncRNA profile of EACSCC and determine the clinical application. Differential expression genes (DEGs) were investigated in EACSCC by whole transcriptome lncRNA arrays (GPL23178). RT-PCR was used to quantify the microarray data. Bioinformatics analyses were performed to evaluate DEGs regulations in gene ontology and cellular pathways. Fluorescence in situ hybridization (FISH) was utilized to validate lncRNA expression. The overall survival was determined by Kaplan-Meier and log-rank analyses. Our microarrays data had been submitted to Gene Expression Omnibus (GSE98912). We identified 5621 DEGs (3185 mRNAs, 2436 lncRNAs) in EACSCC. Lnc-MMP3-1 was the top one upregulated lncRNA in EACSCC with fold change of 237.2 (P < 0.001). RT-PCR results showed similar expression levels as microarrays data. Bioinformatics analyses indicated development of EACSCC was involved in aberrant alternations of multiple biological processes and cellular pathways. FSIH assays also found lnc-MMP3-1 was significantly differentially overexpressed in EACSCC (P < 0.001). Tumor lnc-MMP3-1 levels were closely associated with differentiation degree (P = 0.016), tumor invasion (P = 0.015) and TNM stage (P = 0.015). Moreover, lnc-MMP3-1 expression was a significant prognostic factor in EACSCC (χ

Indexed as

AdultAgedBiomarkers, TumorCarcinoma, Squamous CellComputational BiologyEar CanalEar NeoplasmsFemaleGene Expression ProfilingGene Expression Regulation, NeoplasticGene OntologyHumansMaleMiddle AgedNeoplasm GradingNeoplasm InvasivenessBiomarkers, TumorBioinformaticsexternal auditory canal squamous cell carcinomagene expression assaylncRNAoutcome

Identifiers

PMID28960885
PMCPMC5673923
OpenAlexW2757481839

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.