Evidence map›Paper›PMID 28959661›Full record

ArticleToxicology reports2017

Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep.

Efthalia Kerasioti, Zoi Terzopoulou, Ourania Komini, Ioannis Kafantaris, Sotiria Makri, Dimitrios Stagos, Konstantinos Gerasopoulos, Nikita Y Anisimov, Aristides M Tsatsakis, Demetrios Kouretas

Open access · goldAbstract read
In one paragraph

Article in Toxicology reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 35 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Efthalia KerasiotiDepartment of Biochemistry-Biotechnology, University of Thessaly, Larissa 41500, Greece.
Zoi TerzopoulouDepartment of Biochemistry-Biotechnology, University of Thessaly, Larissa 41500, Greece.
Ourania KominiDepartment of Biochemistry-Biotechnology, University of Thessaly, Larissa 41500, Greece.
Ioannis KafantarisDepartment of Biochemistry-Biotechnology, University of Thessaly, Larissa 41500, Greece.
Sotiria MakriDepartment of Biochemistry-Biotechnology, University of Thessaly, Larissa 41500, Greece.
Dimitrios StagosDepartment of Biochemistry-Biotechnology, University of Thessaly, Larissa 41500, Greece.
Konstantinos GerasopoulosDepartment of Biochemistry-Biotechnology, University of Thessaly, Larissa 41500, Greece.
Nikita Y AnisimovSchool of Engineering, Federal Far East University, Russia.
Aristides M TsatsakisDepartment of Forensic Sciences and Toxicology, Medical School, University of Crete, Heraklion 71003, Greece.
Demetrios KouretasDepartment of Biochemistry-Biotechnology, University of Thessaly, Larissa 41500, Greece.
University of Thessaly · GRFar Eastern Federal University · RUUniversity of Crete · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present study was to investigate the effects of livestock feed supplemented with grape pomace (GP) or olive oil mill wastewater (OMW) byproducts on the enzymatic activity and protein expression of antioxidants enzymes, in liver and spleen tissue of sheep. Thus, 36 male sheep of Chios breed were divided into 3 homogeneous groups, control group (n = 12), GP group (n = 12) and OMW group (n = 12), receiving standard or experimental feed. Liver and spleen tissues were collected at 42 and 70 days post-birth. The enzymatic activity of superoxide dismutase (SOD) and glutathione-s-transferase (GST) and also the protein expression of γ-synthase glutamyl custeine (γ-GCS) were determined in these tissues. The results showed GP group exhibited increased enzymatic activity of GST and protein expression of γ-GCS in liver compared to control group. In GP group's spleen, GST activity was increased compared to control but γ-GCS expression was not affected. In OMW group's liver, GST activity was increased and γ-GCS expression was reduced compared to control. In OMW group's spleen, GST activity was increased but GCS expression was not affected. SOD activity was not affected in both tissues either in GP or OMW group.

Indexed as

AREs, antioxidant response elementsCDNB, 1 chloro-2,4-dinitrobenzeneDETAPAC, diethylenetriaminepentaacetic acidDTT, dithiothreitolGAPDH, glyceraldehyde 3-phosphatedehydrogenaseGlutathione-s-transferase (GST)GP, grape pomaceGrape pomace (GP)GS, glutathione synthaseGSH, glutathioneGST, glutathione-s-transferaseHEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acidKCl, potassium chlorideKeap1, kelch-like ECH-associated protein 1KOH, potassium hydroxideMgCl2, magnesium chlorideNaCl, sodium chlorideNBT, nitroblue tetrazoliumNrf2, nuclear factor-like 2Olive oil mill wastewater (OMW)OMW, olive oil mill wastewaterPVDF, polyvinylidene difluoride membranesROS, reactive oxygen speciesSDS, sodium dodecyl sulfateSOD, superoxide dismutaseSuperoxide dismutase (SOD)XO, xanthine oxidaseγ-GCS, γ-synthase glutamyl cysteineγ-synthase glutamyl custeine (γ-GCS)

Identifiers

PMID28959661
PMCPMC5615154
OpenAlexW2651229095

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.