ArticleIranian journal of biotechnology2015
Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187.
Article in Iranian journal of biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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3 authors at 2 institutions in 1 country.
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Abstract
backgroundThe advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including
objectivesHeterologous protein expression and purification of the full length MATERIALS AND
methodsReverse transcription Polymerase Chain Reaction (RT-PCR) was carried out to amplify an 801 bp fragment of the CP gene from PVY-infected potato leaves. The amplicon was cloned into pGEM-T Easy. The cloned fragment was restricted by
resultsSDS-PAGE analysis of protein from the induced bacteria showed a ~35 KDa protein corresponding to PVY CP. Expression of the recombinant protein was confirmed by anti-His anitibody.
conclusionsThe full-length cDNA of PVY-CP was amplified from the infected potato leaves. The cDNA was heterologously expressed in
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