Evidence map›Paper›PMID 28959293›Full record

ArticleIranian journal of biotechnology2015

Selectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDD

Shiva Hamzeh, Mostafa Motallebi, Mohammad Reza Zamani, Zahra Moghaddassi Jahromi

Abstract read
In one paragraph

Article in Iranian journal of biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shiva HamzehDepartment of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Mostafa MotallebiDepartment of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Mohammad Reza ZamaniDepartment of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Zahra Moghaddassi JahromiDepartment of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSelectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Elimination of SMGs from transgenic crops both increases public acceptance of GM crops and prepares gene stacking possibility for improvement of complex traits. Synthetic inducible promoters provide an efficient and flexible strategy to regulate transgene expression.

objectivesThis study aimed to construct a transformation vector based on Cre/ MATERIALS AND

methodsIn pG-IPFFDD-cre

resultsThe results showed that both

conclusionsThe established system is not only an efficient tool for marker gene elimination but also provides possibility for inducible expression of the transgene.

Indexed as

Cis-acting elementsInducible promoterSelectable marker geneSelf-excision system

Identifiers

PMID28959293
PMCPMC5435017

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.