ArticlePloS one2017
Uncovering the DNA methylation landscape in key regulatory regions within the FADS cluster.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Influence of fatty acid desaturase (FADS) genotype on maternal and child polyunsaturated fatty acids (PUFA) status and child health outcomes: a systematic review.Nutrition reviews · 2020Pooled it
- Childhood fish oil supplementation modifies associations between traffic related air pollution and allergic sensitisation.Environmental health : a global access science source · 2018Trial
- Molecular Insights into the Functional Roles of Variants in the <italic>FADS</italic> Gene Cluster on Omega-3 Long-Chain Polyunsaturated Fatty Acid Synthesis.Lifestyle genomics · 2026Review
- Maternal DHA-rich n-3 PUFAs supplementation interacts with FADS genotypes to influence the profiles of PUFAs in the colostrum among Chinese Han population: a birth cohort study.Nutrition & metabolism · 2022Article
- Existing knowledge on Zn status biomarkers (1963-2021) with a particular focus on FADS1 and FADS2 diagnostic performance and recommendations for further research.Frontiers in nutrition · 2022Review
- Maternal and child fatty acid desaturase genotype as determinants of cord blood long-chain PUFA (LCPUFA) concentrations in the Seychelles Child Development Study.The British journal of nutrition · 2021Article
- Genomics of Postprandial Lipidomics in the Genetics of Lipid-Lowering Drugs and Diet Network Study.Nutrients · 2021Article
- DNA methylation and lipid metabolism: an EWAS of 226 metabolic measures.Clinical epigenetics · 2021Article
- Polyunsaturated fatty acid biosynthesis pathway determines ferroptosis sensitivity in gastric cancer.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Pilot Study Assessing the Impact of rs174537 on Circulating Polyunsaturated Fatty Acids and the Inflammatory Response in Patients with Traumatic Brain Injury.Journal of neurotrauma · 2020Observational
- Review
- FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression.International journal of obesity (2005) · 2019Article
- Liver DNA methylation of FADS2 associates with FADS2 genotype.Clinical epigenetics · 2019Article
- Arachidonic acid and colorectal adenoma risk: a Mendelian randomization study.Clinical epidemiology · 2019Article
- Effect of gestational oily fish intake on the risk of allergy in children may be influenced byGenes & nutrition · 2019Article
- FADS1-FADS2 genetic polymorphisms are associated with fatty acid metabolism through changes in DNA methylation and gene expression.Clinical epigenetics · 2018Article
- Allele-specific methylation in theClinical epigenetics · 2018Article
- Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Genetic variants near and within the fatty acid desaturase (FADS) cluster are associated with polyunsaturated fatty acid (PUFA) biosynthesis, levels of several disease biomarkers and risk of human disease. However, determining the functional mechanisms by which these genetic variants impact PUFA levels remains a challenge. Utilizing an Illumina 450K array, we previously reported strong allele-specific methylation (ASM) associations (p = 2.69×10-29) between a single nucleotide polymorphism (SNP) rs174537 and DNA methylation of CpG sites located in the putative enhancer region between FADS1 and FADS2, in human liver tissue. However, this array only featured 20 CpG sites within this 12kb region. To better understand the methylation landscape within this region, we conducted bisulfite sequencing of the region between FADS1 and FADS2. Liver tissues from 50 male subjects (27 European Americans, 23 African Americans) were obtained from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study, and used to ascertain the genotype at rs174537 and methylation status across the region of interest. Associations between rs174537 genotype and methylation status of 136 CpG sites were determined. Age-adjusted linear regressions were used to assess ASM associations with rs174537 genotype. The majority of CpG sites (117 out of 136, 86%) exhibited high levels of methylation with the greatest variability observed at three key regulatory regions-the promoter regions for FADS1 and FADS2 and a putative enhancer site between the two genes. Eight CpG sites within the putative enhancer region displayed significant (FDR p <0.05) ASM associations with rs174537. These data support the concept that both genetic and epigenetic factors regulate PUFA biosynthesis, and raise fundamental questions as to how genetic variants such as rs174537 impact DNA methylation in distant regulatory regions, and ultimately the capacity of tissues to synthesize PUFAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.