ArticleJournal of virology2017
Genome-Wide Mutagenesis of Dengue Virus Reveals Plasticity of the NS1 Protein and Enables Generation of Infectious Tagged Reporter Viruses.
Article in Journal of virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- A Replication-Competent Flavivirus Genome with a Stable GFP Insertion at the NS1-NS2A Junction.Biology · 2026Article
- Coatomer protein complex I is required for efficient secretion of dengue virus non-structural protein 1.Journal of virology · 2025Article
- Review
- Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies.Virologica Sinica · 2025Article
- Genomic hotspots in the DENV-2 serotype (E, NS4B, and NS5 genes) are associated with dengue disease severity in the endemic region of India.PLoS neglected tropical diseases · 2025Article
- The NS1 protein of contemporary West African Zika virus potentiates viral replication and reduces innate immune activation.PLoS neglected tropical diseases · 2024Article
- AntiviralmBio · 2024Article
- Identification and validation of ferroptosis-related genes in patients infected with dengue virus: implication in the pathogenesis of DENV.Virus genes · 2023Article
- Identification of Key Residues in Dengue Virus NS1 Protein That Are Essential for Its Secretion.Viruses · 2023Article
- The cargo adapter protein CLINT1 is phosphorylated by the Numb-associated kinase BIKE and mediates dengue virus infection.The Journal of biological chemistry · 2022Article
- Genome-Wide CRISPR Screen Identifies RACK1 as a Critical Host Factor for Flavivirus Replication.Journal of virology · 2021Article
- Flavivirus Persistence in Wildlife Populations.Viruses · 2021Review
- Applications of Deep Mutational Scanning in Virology.Viruses · 2021Review
- Conserved epitopes with high HLA-I population coverage are targets of CD8Scientific reports · 2020Article
- Generation of a reporter yellow fever virus for high throughput antiviral assays.Antiviral research · 2020Article
- Identification of Estrogen Receptor Modulators as Inhibitors of Flavivirus Infection.Antimicrobial agents and chemotherapy · 2020Article
- Genome-Wide Mutagenesis of Hepatitis C Virus Reveals Ability of Genome To Overcome Detrimental Mutations.Journal of virology · 2020Article
- Development of HiBiT-Tagged Recombinant Infectious Bronchitis Coronavirus for EfficientFrontiers in microbiology · 2020Article
- Article
- Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein.Journal of virology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dengue virus (DENV) is a major global pathogen that causes significant morbidity and mortality in tropical and subtropical areas worldwide. An improved understanding of the regions within the DENV genome and its encoded proteins that are required for the virus replication cycle will expedite the development of urgently required therapeutics and vaccines. We subjected an infectious DENV genome to unbiased insertional mutagenesis and used next-generation sequencing to identify sites that tolerate 15-nucleotide insertions during the virus replication cycle in hepatic cell culture. This revealed that the regions within capsid, NS1, and the 3' untranslated region were the most tolerant of insertions. In contrast, prM- and NS2A-encoding regions were largely intolerant of insertions. Notably, the multifunctional NS1 protein readily tolerated insertions in regions within the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.