Evidence map›Paper›PMID 28955815›Full record

ArticleBiochemistry and biophysics reports2016

Hydroxylation of methylated DNA by TET1 in chondrocyte differentiation of C3H10T1/2 cells.

Ryo Ito, Hiroki Shimada, Kengo Yazawa, Ikuko Sato, Yuuki Imai, Akira Sugawara, Atsushi Yokoyama

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry and biophysics reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ryo ItoDepartment of Molecular Endocrinology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Hiroki ShimadaDepartment of Molecular Endocrinology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Kengo YazawaDepartment of Molecular Endocrinology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Ikuko SatoDepartment of Molecular Endocrinology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Yuuki ImaiDivision of Integrative Pathophysiology, Proteo-Science Center, Graduate School of Medicine, Ehime University, Ehime, Japan.
Akira SugawaraDepartment of Molecular Endocrinology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Atsushi YokoyamaDepartment of Molecular Endocrinology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Tohoku University · JPEhime University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA methylation is closely involved in the regulation of cellular differentiation, including chondrogenic differentiation of mesenchymal stem cells. Recent studies showed that Ten-eleven translocation (TET) family proteins converted 5-methylcytosine (5mC) to 5-hydroxymethylcytosine, 5-formylcytosine and 5carboxylcytosine by oxidation. These reactions constitute potential mechanisms for active demethylation of methylated DNA. However, the relationship between the DNA methylation patterns and the effects of TET family proteins in chondrocyte differentiation is still unclear. In this study, we showed that DNA hydroxylation of 5mC was increased during chondrocytic differentiation of C3H10T1/2 cells and that the expression of Tet1 was particularly enhanced. Moreover, knockdown experiments revealed that the downregulation of Tet1 expression caused decreases in chondrogenesis markers such as type 2 and type 10 collagens. Furthermore, we found that TET proteins had a site preference for hydroxylation of 5mC on the

Indexed as

5hmC, 5-hydroxymethylcytosine5mC, 5-methylcytosineChondrocyte differentiationCol10Col10, collagen 10Col2Col2, collagen 2HydroxymethylcytosineIgf1Igf1, insulin-like growth factorTET1Tet, ten–eleven translocation

Identifiers

PMID28955815
PMCPMC5600463
OpenAlexW2212927930

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.