Evidence map›Paper›PMID 28955452›Full record

ArticleFungal biology and biotechnology2014

Next-generation genome sequencing can be used to rapidly characterise sequences flanking T-DNA insertions in random insertional mutants of

Kylie Chambers, Rohan Gt Lowe, Barbara J Howlett, Manuel Zander, Jacqueline Batley, Angela P Van de Wouw, Candace E Elliott

Abstract readCase Reports
In one paragraph

Article in Fungal biology and biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kylie ChambersSchool of Botany, the University of Melbourne, Parkville, 3010 Victoria Australia.
Rohan Gt LoweSchool of Botany, the University of Melbourne, Parkville, 3010 Victoria Australia.
Barbara J HowlettSchool of Botany, the University of Melbourne, Parkville, 3010 Victoria Australia.
Manuel ZanderSchool of Agriculture and Food Sciences, University of Queensland, St Lucia, Brisbane, 4072 Queensland Australia.
Jacqueline BatleySchool of Agriculture and Food Sciences, University of Queensland, St Lucia, Brisbane, 4072 Queensland Australia.
Angela P Van de WouwSchool of Botany, the University of Melbourne, Parkville, 3010 Victoria Australia.
Candace E ElliottSchool of Botany, the University of Melbourne, Parkville, 3010 Victoria Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBanks of mutants with random insertions of T-DNA from

findingsNext-generation Illumina sequencing was used to locate T-DNA insertion sites in four mutants of

conclusionsNext-generation sequencing is a cost-effective and rapid method of identifying sites of T-DNA insertions, and associated genomic rearrangements in

Indexed as

Filamentous fungiNext-generation sequencingTAIL-PCRT-DNA mutants

Identifiers

PMID28955452
PMCPMC5611616

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.