ArticleBosnian journal of basic medical sciences2018
Physiological and transcriptomic analysis of a salt-resistant Saccharomyces cerevisiae mutant obtained by evolutionary engineering.
Article in Bosnian journal of basic medical sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 28 citations in OpenAlex.
- Transcriptomics-guided identification of engineering targets for improving salt tolerance in Saccharomyces cerevisiae.Microbial cell factories · 2026Article
- Biofilm-based continuous secretion of human lysozyme through cell-surface display of FimH onSynthetic and systems biotechnology · 2026Article
- Mechanism of enhanced salt tolerance in Saccharomyces cerevisiae by CRZ1 overexpression.Scientific reports · 2024Article
- Comparative transcriptome analysis of doramectin-producing Streptomyces avermitilis N72 and its mutant strains.World journal of microbiology & biotechnology · 2024Article
- Performance and robustness analysis reveals phenotypic trade-offs in yeast.Life science alliance · 2024Article
- Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation.Biotechnology for biofuels and bioproducts · 2023Article
- Comparative Transcriptome Analysis of Two Chrysomycin-Producing Wild-Type and Mutant Strains ofMetabolites · 2022Article
- Transcriptome Analysis Reveals that MAPK Signaling Pathway Mediates Salt Tolerance of YMR253C ORF in Saccharomyces cerevisiae.Current microbiology · 2022Article
- Seven Years at High Salinity-Experimental Evolution of the Extremely Halotolerant Black YeastJournal of fungi (Basel, Switzerland) · 2021Article
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- In vitro activity of four triazole antifungal drugs against clinically common and uncommon yeast species.Current medical mycology · 2019Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salt-resistant yeast strains are highly demanded by industry due to the exposure of yeast cells to high concentrations of salt, in various industrial bioprocesses. The aim of this study was to perform a physiological and transcriptomic analysis of a salt-resistant Saccharomyces cerevisiae (S. cerevisiae) mutant generated by evolutionary engineering. NaCl-resistant S. cerevisiae strains were obtained by ethyl methanesulfonate (EMS) mutagenesis followed by successive batch cultivations in the presence of gradually increasing NaCl concentrations, up to 8.5% w/v of NaCl (1.45 M). The most probable number (MPN) method, high-performance liquid chromatography (HPLC), and glucose oxidase/peroxidase method were used for physiological analysis, while Agilent yeast DNA microarray systems were used for transcriptome analysis. NaCl-resistant mutant strain T8 was highly cross-resistant to LiCl and highly sensitive to AlCl3. In the absence of NaCl stress, T8 strain had significantly higher trehalose and glycogen levels compared to the reference strain. Global transcriptome analysis by means of DNA microarrays showed that the genes related to stress response, carbohydrate transport, glycogen and trehalose biosynthesis, as well as biofilm formation, were upregulated. According to gene set enrichment analysis, 548 genes were upregulated and 22 downregulated in T8 strain, compared to the reference strain. Among the 548 upregulated genes, the highest upregulation was observed for the FLO11 (MUC1) gene (92-fold that of the reference strain). Overall, evolutionary engineering by chemical mutagenesis and increasing NaCl concentrations is a promising approach in developing industrial strains for biotechnological applications.
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