Evidence map›Paper›PMID 28953884›Full record

ArticleNature2017

Genome editing reveals a role for OCT4 in human embryogenesis.

Norah M E Fogarty, Afshan McCarthy, Kirsten E Snijders, Benjamin E Powell, Nada Kubikova, Paul Blakeley, Rebecca Lea, Kay Elder, Sissy E Wamaitha, Daesik Kim and 8 more

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 181 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
181citing papers in PubMed, 2 pooled it
21.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

181 citing papers in PubMed, 2 syntheses or guidelines pooled it, 392 citations in OpenAlex.

  1. Pooled it
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  12. Species-specific roles of SP1 in bovine and human embryonic genome activation and early embryonic development.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  13. Clocks and Dominoes: Timing Mechanisms of Embryogenesis.bioRxiv : the preprint server for biology · 2026
    Article
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121 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors at 8 institutions in 2 countries.

Norah M E FogartyHuman Embryo and Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
Afshan McCarthyHuman Embryo and Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
Kirsten E SnijdersNIHR Cambridge Biomedical Research Centre hIPSC Core Facility, Department of Surgery, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0SZ, UK.
Benjamin E PowellSex Chromosome Biology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Nada KubikovaNuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK.
Paul BlakeleyHuman Embryo and Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
Rebecca LeaHuman Embryo and Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
Kay ElderBourn Hall Clinic, Bourn, Cambridge CB23 2TN, UK.
Sissy E WamaithaHuman Embryo and Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
Daesik KimDepartment of Chemistry, Seoul National University, Seoul 151-747, South Korea.
Valdone MaciulyteSex Chromosome Biology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Jens KleinjungBioinformatics Facility, The Francis Crick Institute, London NW1 1AT, UK.
Jin-Soo KimDepartment of Chemistry, Seoul National University, Seoul 151-747, South Korea.
Dagan WellsNuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK.
Ludovic VallierNIHR Cambridge Biomedical Research Centre hIPSC Core Facility, Department of Surgery, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0SZ, UK.
Alessandro BerteroWellcome Trust and MRC Cambridge Stem Cell Institute and Biomedical Research Centre, Anne McLaren Laboratory, Department of Surgery, University of Cambridge, Cambridge CB2 0SZ, UK.
James M A TurnerSex Chromosome Biology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Kathy K NiakanHuman Embryo and Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
The Francis Crick Institute · GBSeoul National University · KRBourn Hall Clinic · GBJohn Radcliffe Hospital · GBNIHR Southampton Biomedical Research Centre · GBUniversity of Oxford · GBWellcome/MRC Cambridge Stem Cell Institute · GBWellcome Sanger Institute · GB

Funding

British Heart Foundation FS/11/77/39327Cancer Research UK FC001193Medical Research Council MC_PC_12009Wellcome Trust FC001120Wellcome Trust FC001193
6 · The paper itself

Abstract

Despite their fundamental biological and clinical importance, the molecular mechanisms that regulate the first cell fate decisions in the human embryo are not well understood. Here we use CRISPR-Cas9-mediated genome editing to investigate the function of the pluripotency transcription factor OCT4 during human embryogenesis. We identified an efficient OCT4-targeting guide RNA using an inducible human embryonic stem cell-based system and microinjection of mouse zygotes. Using these refined methods, we efficiently and specifically targeted the gene encoding OCT4 (POU5F1) in diploid human zygotes and found that blastocyst development was compromised. Transcriptomics analysis revealed that, in POU5F1-null cells, gene expression was downregulated not only for extra-embryonic trophectoderm genes, such as CDX2, but also for regulators of the pluripotent epiblast, including NANOG. By contrast, Pou5f1-null mouse embryos maintained the expression of orthologous genes, and blastocyst development was established, but maintenance was compromised. We conclude that CRISPR-Cas9-mediated genome editing is a powerful method for investigating gene function in the context of human development.

Indexed as

Gene EditingGene Expression Regulation, DevelopmentalAnimalsBlastocystCell LineageCRISPR-Cas SystemsEctodermEmbryo, MammalianEmbryonic DevelopmentFemaleGerm LayersHuman Embryonic Stem CellsHumansMaleMiceNanog Homeobox ProteinNanog Homeobox ProteinOctamer Transcription Factor-3POU5F1 protein, human

Identifiers

PMID28953884
PMCPMC5815497
OpenAlexW2759601393

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.