ArticleLab on a chip2017
A microfluidic trap array for longitudinal monitoring and multi-modal phenotypic analysis of individual stem cell aggregates.
Article in Lab on a chip, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Self-Regulated Selective Surface Coating Enables Confinement of Adherent Cells in Closed Microfluidic Arrays.Analytical chemistry · 2025Article
- Generation of 3D Spheroids Using a Thiol-Acrylate Hydrogel Scaffold to Study Endocrine Response in ERACS biomaterials science & engineering · 2022Article
- Rapid Prototyping of Organ-on-a-Chip Devices Using Maskless Photolithography.Micromachines · 2021Article
- High-Temporal-Resolution smFISH Method for Gene Expression Studies inAnalytical chemistry · 2021Article
- Microdissected "cuboids" for microfluidic drug testing of intact tissues.Lab on a chip · 2021Article
- Sequential storage and release of microdroplets.Microsystems & nanoengineering · 2021Article
- Microfluidic perfusion modulates growth and motor neuron differentiation of stem cell aggregates.The Analyst · 2020Article
- Population-based analysis of cell-penetrating peptide uptake using a microfluidic droplet trapping array.Analytical and bioanalytical chemistry · 2019Article
- Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics.Molecular biotechnology · 2018Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Three-dimensional pluripotent stem cell (PSC) cultures have the ability to undergo differentiation, self-organization, and morphogenesis to yield complex, in vitro tissue models that recapitulate key elements of native tissues. These tissue models offer a system for studying mechanisms of tissue development, investigating disease mechanisms, and performing drug screening. It remains challenging, however, to standardize PSC aggregate differentiation and morphogenesis methods due to heterogeneity stemming from biological and environmental sources. It is also difficult to monitor and assess large numbers of individual samples longitudinally throughout culture using typical batch-based culture methods. To address these challenges, we have developed a microfluidic platform for culture, longitudinal monitoring, and phenotypic analysis of individual stem cell aggregates. This platform uses a hydrodynamic loading principle to capture pre-formed stem cell aggregates in independent traps. We demonstrated that multi-day culture of aggregates in this platform reduces heterogeneity in phenotypic parameters such as size and morphology. Additionally, we showed that culture and analysis steps can be performed sequentially in the same platform, enabling correlation of multiple modes of analysis for individual samples. We anticipate this platform being applied to improve abilities for phenotypic analysis of PSC aggregate tissues and to facilitate research in standardizing culture systems in order to dually increase the yield and reduce the heterogeneity of PSC-derived tissues.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.