ArticleBioengineering (Basel, Switzerland)2017
Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture.
Article in Bioengineering (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Design and validation of a perfusion bioreactor for long-term human bone explant culture.JBMR plus · 2026Article
- Design and Performance Evaluation of TPMS-Based Dual-Layer Gradient Porous Structures for Bone Scaffolds.Journal of functional biomaterials · 2026Article
- Design and Validation of a Multi-Modal Bioreactor System: Assessing the Effects of Perfusion and Cyclic Tensile Stimulation on Mechanical and Biological Properties of 3D-Printed Missing-Rib Auxetic Scaffolds.Bioengineering (Basel, Switzerland) · 2026Article
- 3D cell culture model - a substitution for future in vivo fish study?Molecular biology reports · 2025Review
- Development of a novel high-throughput culture system for hypoxic 3D hydrogel cell culture.Scientific reports · 2024Article
- Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system.Biotechnology letters · 2024Article
- Mechanical and Computational Fluid Dynamic Models for Magnesium-Based Implants.Materials (Basel, Switzerland) · 2024Article
- Article
- Preclinical Research of Stem Cells: Challenges and Progress.Stem cell reviews and reports · 2023Review
- Design and 3D Printing of Personalized Hybrid and Gradient Structures for Critical Size Bone Defects.ACS applied bio materials · 2023Article
- Geometry-Based Computational Fluid Dynamic Model for Predicting the Biological Behavior of Bone Tissue Engineering Scaffolds.Journal of functional biomaterials · 2022Article
- Optimization and Validation of a Custom-Designed Perfusion Bioreactor for Bone Tissue Engineering: Flow Assessment and Optimal Culture Environmental Conditions.Frontiers in bioengineering and biotechnology · 2022Article
- Influence of Charge and Heat on the Mechanical Properties of Scaffolds from Ionic Complexation of Chitosan and Carboxymethyl Cellulose.ACS biomaterials science & engineering · 2021Article
- Finite element study of stem cells under fluid flow for mechanoregulation toward osteochondral cells.Journal of materials science. Materials in medicine · 2021Article
- 3D Printing of Cell Culture Devices: Assessment and Prevention of the Cytotoxicity of Photopolymers for Stereolithography.Materials (Basel, Switzerland) · 2020Article
- Strategy for achieving standardized bone models.Biotechnology and bioengineering · 2020Review
- A Perfusion Bioreactor System for Cell Seeding and Oxygen-Controlled Cultivation of Three-Dimensional Cell Cultures.Tissue engineering. Part C, Methods · 2018Article
- FABRICA: A Bioreactor Platform for Printing, Perfusing, Observing, & Stimulating 3D Tissues.Scientific reports · 2018Article
- Numerical Evaluation and Prediction of Porous Implant Design and Flow Performance.BioMed research international · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The three dimensional (3D) cultivation of stem cells in dynamic bioreactor systems is essential in the context of regenerative medicine. Still, there is a lack of bioreactor systems that allow the cultivation of multiple independent samples under different conditions while ensuring comprehensive control over the mechanical environment. Therefore, we developed a miniaturized, parallelizable perfusion bioreactor system with two different bioreactor chambers. Pressure sensors were also implemented to determine the permeability of biomaterials which allows us to approximate the shear stress conditions. To characterize the flow velocity and shear stress profile of a porous scaffold in both bioreactor chambers, a computational fluid dynamics analysis was performed. Furthermore, the mixing behavior was characterized by acquisition of the residence time distributions. Finally, the effects of the different flow and shear stress profiles of the bioreactor chambers on osteogenic differentiation of human mesenchymal stem cells were evaluated in a proof of concept study. In conclusion, the data from computational fluid dynamics and shear stress calculations were found to be predictable for relative comparison of the bioreactor geometries, but not for final determination of the optimal flow rate. However, we suggest that the system is beneficial for parallel dynamic cultivation of multiple samples for 3D cell culture processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.