ArticleBioengineering (Basel, Switzerland)2017
Fed-Batch Synthesis of Poly(3-Hydroxybutyrate) and Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) from Sucrose and 4-Hydroxybutyrate Precursors by Burkholderia sacchari Strain DSM 17165.
Article in Bioengineering (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 60 citations in OpenAlex.
- Polyhydroxybutyrate (PHB): Critical Perspectives on Material Properties, Production Advances, and Challenges Toward Sustainable Commercialisation.Materials (Basel, Switzerland) · 2026Review
- Sucrose-Based Screening of a Novel Strain,Polymers · 2025Article
- The Role of Bacterial Polyhydroalkanoate (PHA) in a Sustainable Future: A Review on the Biological Diversity.International journal of environmental research and public health · 2023Review
- Biosynthesis of Poly-(3-hydroxybutyrate) under the Control of an Anaerobically Induced Promoter by RecombinantMolecules (Basel, Switzerland) · 2022Article
- Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks.Frontiers in bioengineering and biotechnology · 2022Review
- Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production.Molecules (Basel, Switzerland) · 2021Review
- PHA Production and PHA Synthases of the Halophilic BacteriumBioengineering (Basel, Switzerland) · 2020Article
- Review
- Biomedical Processing of Polyhydroxyalkanoates.Bioengineering (Basel, Switzerland) · 2019Review
- (Bio)degradable Polymeric Materials for Sustainable Future-Part 2: Degradation Studies of P(3HB-Polymers · 2019Article
- Investigating Nutrient Limitation Role on Improvement of Growth and Poly(3-Hydroxybutyrate) Accumulation byFrontiers in bioengineering and biotechnology · 2019Article
- Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity.Polymers · 2018Review
- xylA and xylB overexpression as a successful strategy for improving xylose utilization and poly-3-hydroxybutyrate production in Burkholderia sacchari.Journal of industrial microbiology & biotechnology · 2018Article
- Biodegradable and Biocompatible Polyhydroxy-alkanoates (PHA): Auspicious Microbial Macromolecules for Pharmaceutical and Therapeutic Applications.Molecules (Basel, Switzerland) · 2018Review
- Advances in Polyhydroxyalkanoate (PHA) Production.Bioengineering (Basel, Switzerland) · 2017Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on direct sucrose conversion, the bacterium Burkholderia sacchari is an excellent producer of the microbial homopolyester poly(3-hydroxybutyrate) (PHB). Restrictions of the strain's wild type in metabolizing structurally related 3-hydroxyvalerate (3HV) precursors towards 3HV-containing polyhydroxyalkanoate (PHA) copolyester calls for alternatives. We demonstrate the highly productive biosynthesis of PHA copolyesters consisting of 3-hydroxybuytrate (3HB) and 4-hydroxybutyrate (4HB) monomers. Controlled bioreactor cultivations were carried out using saccharose from the Brazilian sugarcane industry as the main carbon source, with and without co-feeding with the 4HB-related precursor γ-butyrolactone (GBL). Without GBL co-feeding, the homopolyester PHB was produced at a volumetric productivity of 1.29 g/(L•h), a mass fraction of 0.52 g PHB per g biomass, and a final PHB concentration of 36.5 g/L; the maximum specific growth rate µmax amounted to 0.15 1/h. Adding GBL, we obtained 3HB and 4HB monomers in the polyester at a volumetric productivity of 1.87 g/(L•h), a mass fraction of 0.72 g PHA per g biomass, a final PHA concentration of 53.7 g/L, and a µmax of 0.18 1/h. Thermoanalysis revealed improved material properties of the second polyester in terms of reduced melting temperature Tm (161 °C vs. 178 °C) and decreased degree of crystallinity Xc (24% vs. 71%), indicating its enhanced suitability for polymer processing.
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