ArticleApplied microbiology and biotechnology2017
Metabolic characterization of a CHO cell size increase phase in fed-batch cultures.
Article in Applied microbiology and biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 137 citations in OpenAlex.
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- Comparing kinetic versus stoichiometric priorities in hybrid models of CHO metabolism.NPJ systems biology and applications · 2026Article
- Selecting methods for draft GEM generation in multicellular eukaryotes: a comparative analysis.BMC bioinformatics · 2026Article
- Eicosadienoic acid enhances antibody production in CHO cells by prolonging culture longevity and attenuating apoptosis.AMB Express · 2026Article
- Lab-Scale Continuous Biomanufacturing: A Tool for Process Development With Adaptive Strategies for Capture and Virus Inactivation.Biotechnology and bioengineering · 2026Article
- Comprehensive Lipidomic Analysis of Recombinant Protein-Producing CHO Cell Cultures: Insights for Media and Process Development.Mass spectrometry (Tokyo, Japan) · 2026Article
- Impact of keto leucine and isoleucine on CHO cell central carbon metabolism and performance in fed-batch and steady-state perfusion.Frontiers in bioengineering and biotechnology · 2026Article
- Glycolytic ATP production enables rapid mammalian cell growth.bioRxiv : the preprint server for biology · 2025Article
- Automated smartphone based cell analysis platform.Npj imaging · 2025Article
- Flux Sampling Suggests Metabolic Signatures of High Antibody-Producing CHO Cells.Biotechnology and bioengineering · 2025Article
- Machine Learning-Powered Optimization of a CHO Cell Cultivation Process.Biotechnology and bioengineering · 2025Article
- Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida.Communications biology · 2025Article
- Incorporating shaken 24-deep-well plate fed-batch culture shortens CHO cell line development time.Cytotechnology · 2025Article
- Defining Golden Batches in Biomanufacturing Processes From Internal Metabolic Activity to Detect Process Changes That May Affect Product Quality.Biotechnology and bioengineering · 2025Article
- Viable cell sorting by fluidized bed centrifugation enables novel cultivation strategies.Frontiers in bioengineering and biotechnology · 2025Article
- Eukaryotic cell size regulation and its implications for cellular function and dysfunction.Physiological reviews · 2024Review
- Building blocks needed for mechanistic modeling of bioprocesses: A critical review based on protein production by CHO cells.Metabolic engineering communications · 2024Review
- Deciphering Metabolic Pathways in High-Seeding-Density Fed-Batch Processes for Monoclonal Antibody Production: A Computational Modeling Perspective.Bioengineering (Basel, Switzerland) · 2024Article
- Integrated Experimental and Mathematical Exploration of Modular Tissue Cultures for Developmental Engineering.International journal of molecular sciences · 2024Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Normally, the growth profile of a CHO cell fed-batch process can be divided into two main phases based on changes in cell concentration, being an exponential growth phase and a stationary (non-growth) phase. In this study, an additional phase is observed during which the cell division comes to a halt but the cell growth continues in the form of an increase in cell size. The cell size increase (SI) phase occurs between the exponential proliferation phase (also called the number increase or NI phase) and the stationary phase. During the SI phase, the average volume and dry weight per cell increase threefold linearly with time. The average mAb specific productivity per cell increases linearly with the cell volume and therefore is on average two times higher in the SI phase than in the NI phase. The specific essential amino acids consumption rates per cell remain fairly constant between the NI and the SI phase, which agrees with the similar biomass production rate per cell between these two phases. Accumulation of fatty acids and formation of lipid droplets in the cells are observed during the SI phase, indicating that the fatty acids synthesis rate exceeds the demand for the synthesis of membrane lipids. A metabolic comparison between NI and SI phase shows that the cells with a larger size produce more mAb per unit of O
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