ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2017
[Rapid detection of alpha-globin gene ααα
Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
5 authors.
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Abstract
objectiveTo establish a rapid method for detection of alpha-globin gene ααα
methodsThe differential sequence between the X1 and Y1 box of α1 gene was selected as the amplicon of the target gene with β-actin as the reference gene. The specific primers and TaqMan probes were designed, and then a quantitative method for detecting the copy number was established based on ddPCR technique. The sensitivity and accuracy of the method were evaluated by detecting 28 samples of known genotypes and 60 clinical samples.
resultsThe ddPCR-based method accurately identified the genotypes of all the 28 samples with known genotypes and detected 5 cases of αα/ααα
conclusionThis ddPCR-based method for detecting ααα
Identifiers
28951374PMC6765481What OpenQuestion holds
Registered trials
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