Evidence map›Paper›PMID 28950428›Full record

ArticleAndrology2017

Cigarette smoking significantly alters sperm DNA methylation patterns.

T G Jenkins, E R James, D F Alonso, J R Hoidal, P J Murphy, J M Hotaling, B R Cairns, D T Carrell, K I Aston

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Andrology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed, 4 pooled it
6.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 4 syntheses or guidelines pooled it, 143 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Genome-wide DNA methylation differences in nucleus accumbens of smokers vs. nonsmokers.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2021
    Pooled it
  4. Pooled it
  5. Review
  6. Environmental and Genetic Perturbations of the Sperm Epigenome.Advances in experimental medicine and biology · 2026
    Review
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14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

T G JenkinsDepartment of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA.
E R JamesDepartment of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA.
D F AlonsoDepartment of Psychology, University of Utah, Salt Lake City, UT, USA.
J R HoidalDepartment of Internal Medicine, University of Utah School of Medicine, Salt Lake City, UT, USA.
P J MurphyDepartment of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT, USA.
J M HotalingDepartment of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA.
B R CairnsDepartment of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT, USA.
D T CarrellDepartment of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA.
K I AstonDepartment of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA.ORCID http://orcid.org/0000-0001-6459-2103
University of Utah · USHoward Hughes Medical Institute · US

Funding

Transgenerational Effects of Smoking-Induced Changes to Sperm DNA MethylationR01HD082062 · NICHD · UNIVERSITY OF UTAH · PI ASTON, KENNETH IVAN, CAIRNS, BRADLEY R. · 2015 to 2016
$615k
NICHD NIH HHS R01 HD082062
6 · The paper itself

Abstract

Numerous health consequences of tobacco smoke exposure have been characterized, and the effects of smoking on traditional measures of male fertility are well described. However, a growing body of data indicates that pre-conception paternal smoking also confers increased risk for a number of morbidities on offspring. The mechanism for this increased risk has not been elucidated, but it is likely mediated, at least in part, through epigenetic modifications transmitted through spermatozoa. In this study, we investigated the impact of cigarette smoke exposure on sperm DNA methylation patterns in 78 men who smoke and 78 never-smokers using the Infinium Human Methylation 450 beadchip. We investigated two models of DNA methylation alterations: (i) consistently altered methylation at specific CpGs or within specific genomic regions and (ii) stochastic DNA methylation alterations manifest as increased variability in genome-wide methylation patterns in men who smoke. We identified 141 significantly differentially methylated CpGs associated with smoking. In addition, we identified a trend toward increased variance in methylation patterns genome-wide in sperm DNA from men who smoke compared with never-smokers. These findings of widespread DNA methylation alterations are consistent with the broad range of offspring heath disparities associated with pre-conception paternal smoke exposure and warrant further investigation to identify the specific mechanism by which sperm DNA methylation perturbation confers risk to offspring health and whether these changes can be transmitted to offspring and transgenerationally.

Indexed as

DNA MethylationSpermatozoaAdultCigarette SmokingCpG IslandsHumansMaleepigeneticsgenome-widesmokingsperm DNA methylationtransgenerational inheritance

Identifiers

PMID28950428
PMCPMC5679018
OpenAlexW2759363180

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.