ArticlePloS one2017
Assessment of MALDI-TOF MS biotyping for Borrelia burgdorferi sl detection in Ixodes ricinus.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- [West African tick-borne relapsing fever, a neglected disease of major public health importance in Senegal].Medecine tropicale et sante internationale · 2025Pooled it
- Optimization of tick identification via MALDI-TOF MS for implementation in routine diagnostics.Parasitology research · 2026Article
- Environmental hazard of tick-borne diseases in urban and peri-urban sites in an endemic area of eastern France.Parasite (Paris, France) · 2026Article
- Glossina from the Republic of the Congo: species identification by MALDI-TOF MS and research of associated micro-organisms.Parasite (Paris, France) · 2026Article
- MultiStageSearch: An Iterative Workflow for Unbiased Taxonomic Analysis of Pathogens Using Proteogenomics.Journal of proteome research · 2025Article
- Challenges for ticks and tick-borne diseases research in Southeast Asia: Insight from the first international symposium in Cambodia.PLoS neglected tropical diseases · 2024Review
- Prevalence of Lyme Disease and Relapsing FeverPathogens (Basel, Switzerland) · 2024Review
- Article
- Evolution of MALDI-TOF MS Profiles from Lice and Fleas Preserved in Alcohol over Time.Insects · 2023Article
- Survey of bed bug infestations in homeless shelters in southern France.Scientific reports · 2023Article
- PepGM: a probabilistic graphical model for taxonomic inference of viral proteome samples with associated confidence scores.Bioinformatics (Oxford, England) · 2023Article
- Utility of MALDI-TOF MS for determination of species identity and blood meal sources of primary malaria vectors on the Kenyan coast.Wellcome open research · 2023Article
- Identification and Typing of Strains of Wood-Rotting Basidiomycetes by Protein Profiling Using MALDI-TOF MS.Biotech (Basel (Switzerland)) · 2022Article
- Impact of Different Anthropogenic Environments on Ticks and Tick-Associated Pathogens in Alsace, a French Region Highly Endemic for Tick-Borne Diseases.Microorganisms · 2022Article
- Use of the proteomic tool MALDI-TOF MS in termite identification.Scientific reports · 2022Article
- Complete Genome Sequence ofMicrobiology resource announcements · 2020Article
- A Novel Rapid Sample Preparation Method for MALDI-TOF MS PermitsFrontiers in microbiology · 2020Article
- Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts.PloS one · 2020Article
- Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals.Parasite (Paris, France) · 2020Article
- Identification of closely related Ixodes species by protein profiling with MALDI-TOF mass spectrometry.PloS one · 2019Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been demonstrated to be useful for tick identification at the species level. More recently, this tool has been successfully applied for the detection of bacterial pathogens directly in tick vectors. The present work has assessed the detection of Borrelia burgdorferi sensu lato in Ixodes ricinus tick vector by MALDI-TOF MS. To this aim, experimental infection model of I. ricinus ticks by B. afzelii was carried out and specimens collected in the field were also included in the study. Borrelia infectious status of I. ricinus ticks was molecularly controlled using half-idiosome to classify specimens. Among the 39 ticks engorged on infected mice, 14 were confirmed to be infected by B. afzelii. For field collection, 14.8% (n = 12/81) I. ricinus ticks were validated molecularly as infected by B. burgdorferi sl. To determine the body part allowing the detection of MS protein profile changes between non-infected and B. afzelii infected specimens, ticks were dissected in three compartments (i.e. 4 legs, capitulum and half-idiosome) prior to MS analysis. Highly reproducible MS spectra were obtained for I. ricinus ticks according to the compartment tested and their infectious status. However, no MS profile change was found when paired body part comparison between non-infected and B. afzelii infected specimens was made. Statistical analyses did not succeed to discover, per body part, specific MS peaks distinguishing Borrelia-infected from non-infected ticks whatever their origins, laboratory reared or field collected. Despite the unsuccessful of MALDI-TOF MS to classify tick specimens according to their B. afzelii infectious status, this proteomic tool remains a promising method for rapid, economic and accurate identification of tick species. Moreover, the singularity of MS spectra between legs and half-idiosome of I. ricinus could be used to reinforce this proteomic identification by submission of both these compartments to MS.
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