ArticleAnalytical chemistry2017
Assessment of Extracellular Vesicles Purity Using Proteomic Standards.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Quantitative Analysis of Complement Membrane Attack Complex Proteins Associated with Extracellular Vesicles.Proteomes · 2024Article
- Article
- Extracellular vesicles: Emerged as a promising strategy for regenerative medicine.World journal of stem cells · 2023Review
- Advances in Mass Spectrometry on Membrane Proteins.Membranes · 2023Review
- Isolation protocols and mitochondrial content for plasma extracellular vesicles.Analytical and bioanalytical chemistry · 2023Article
- Recent advances of small extracellular vesicle biomarkers in breast cancer diagnosis and prognosis.Molecular cancer · 2023Review
- Addressing MISEV guidance using targeted LC-MS/MS: A method for the detection and quantification of extracellular vesicle-enriched and contaminant protein markers from blood.Journal of extracellular biology · 2022Article
- Proteomic Profile of Procoagulant Extracellular Vesicles Reflects Complement System Activation and Platelet Hyperreactivity of Patients with Severe COVID-19.Frontiers in cellular and infection microbiology · 2022Article
- The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation.Viruses · 2021Review
- Quantitative Proteomic Analysis of Biogenesis-Based Classification for Extracellular Vesicles.Proteomes · 2020Article
- Proteomics of Extracellular Vesicles: Update on Their Composition, Biological Roles and Potential Use as Diagnostic Tools in Atherosclerotic Cardiovascular Diseases.Diagnostics (Basel, Switzerland) · 2020Review
- Proteomic analysis of circulating extracellular vesicles identifies potential markers of breast cancer progression, recurrence, and response.Science advances · 2020Article
- Plasma-Derived Extracellular Vesicle Phosphoproteomics through Chemical Affinity Purification.Journal of proteome research · 2020Article
- Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry.Journal of extracellular vesicles · 2020Article
- An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids.Molecules (Basel, Switzerland) · 2019Review
- Highly Efficient Phosphoproteome Capture and Analysis from Urinary Extracellular Vesicles.Journal of proteome research · 2018Article
- Proteomic Toolbox To Standardize the Separation of Extracellular Vesicles and Lipoprotein Particles.Journal of proteome research · 2018Article
- Automated Removal of Phospholipids from Membrane Proteins for H/D Exchange Mass Spectrometry Workflows.Analytical chemistry · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The increasing interest in extracellular vesicles (EVs) research is fueled by reports indicating their unique role in intercellular communication and potential connection to the development of common human diseases. The unique role assumes unique protein and nucleic acid cargo. Unfortunately, accurate analysis of EVs cargo faces a challenge of EVs isolation. Generally used isolation techniques do not separate different subtypes of EVs and even more, poorly separate EVs from non-EVs contaminants. Further development of EVs isolation protocols urgently needs a quantitative method of EVs purity assessment. We report here that multiple reaction monitoring assay using internal standards carrying peptides for quantification of EVs and non-EVs proteins is a suitable approach to assess purity of EVs preparations. As a first step in potential standardization of EVs isolation, we have evaluated polymer-based precipitation techniques and compared them to traditional ultracentrifugation protocol.
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Registered trials
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