ArticleAmerican journal of human biology : the official journal of the Human Biology Council2018
Evaluating minimally invasive sample collection methods for telomere length measurement.
Article in American journal of human biology : the official journal of the Human Biology Council, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06357663 (Effect of Subtle Energy Transmission and Tao Calligraphy Mindfulness Practice on Telomere Length in Peripheral Blood Leukocytes A Follow-up Pilot Study), which is not on this map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of Subtle Energy Transmission and Tao Calligraphy Mindfulness Practice on Telomere Length in Peripheral Blood Leukocytes A Follow-up Pilot Study
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Telomere length analysis from minimally-invasively collected samples: Methods development and meta-analysis of the validity of different sampling techniques: American Journal of Human Biology.American journal of human biology : the official journal of the Human Biology Council · 2021Pooled it
- Telehealth Parenting Program and Salivary Epigenetic Biomarkers in Preschool Children With Developmental Delay: NIMHD Social Epigenomics Program.JAMA network open · 2024Trial
- Telomere Length, Oxidative Stress Markers, and Related miRNAs in Non-Invasive Samples of Mild COVID-19 Cases.International journal of molecular sciences · 2025Article
- Temperament and sex as moderating factors of the effects of exposure to maternal depression on telomere length in early childhood.Development and psychopathology · 2025Article
- Unveiling the Impact of Peanut Consumption on Telomere Length in Young and Healthy Individuals: Insights from the ARISTOTLE Study: A Randomized Clinical Trial.Antioxidants (Basel, Switzerland) · 2025Article
- Associations among temperament characteristics and telomere length and attrition rate in early childhood.Developmental psychology · 2024Article
- Cross-tissue comparison of telomere length and quality metrics of DNA among individuals aged 8 to 70 years.PloS one · 2024Article
- Minimally invasive biomarkers in human and non-human primate evolutionary biology: Tools for understanding variation and adaptation.American journal of human biology : the official journal of the Human Biology Council · 2022Article
- No Mediation Effect of Telomere Length or Mitochondrial DNA Copy Number on the Association Between Adverse Childhood Experiences (ACEs) and Central Arterial Stiffness.Journal of the American Heart Association · 2022Article
- Telomere Length and Preterm Birth in Pregnant Mexican-Origin Women.Maternal and child health journal · 2021Article
- From culture to chromosomes: A mother-child dyadic study of acculturation, telomere lengths and body fat.Comprehensive psychoneuroendocrinology · 2021Article
- Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.PloS one · 2021Article
- Maternal psychosocial functioning, obstetric health history, and newborn telomere length.Psychoneuroendocrinology · 2021Article
- Patterns of change in telomere length over the first three years of life in healthy children.Psychoneuroendocrinology · 2020Article
- Stress and Salivary Telomere Length in the Second Half of Life: A Comparison of Life-course Models.Advances in life course research · 2019Article
- Lymphocyte homeostasis is maintained in perinatally HIV-infected patients after three decades of life.Immunity & ageing : I & A · 2019Article
- Telomere length measurement by qPCR - Summary of critical factors and recommendations for assay design.Psychoneuroendocrinology · 2019Review
- A scoping systematic review of social stressors and various measures of telomere length across the life course.Ageing research reviews · 2018Article
- Reproduction predicts shorter telomeres and epigenetic age acceleration among young adult women.Scientific reports · 2018Article
- Does Salivary Telomere Length Explain Race/Ethnic Differences in Aging?Biodemography and social biologyArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
objectivesTelomere length (TL) is a biomarker of aging and age-related decline. Although venous blood is considered the "gold standard" for TL measurement, its collection is often not feasible or desired in nonclinical settings. Saliva and dried blood spots (DBS) have been used as alternatives when venipuncture cannot be performed. However, it is not known whether these sample types yield TL measurements comparable to those obtained from venous blood. We sought to determine whether different samples from the same individual yield comparable TL measurements.
methodsWe extracted DNA from matched buffy coat, saliva (Oragene and Oasis), and DBS (venous and capillary) samples from 40 women aged 18-77 years. We used the monochrome multiplex qPCR (MMQPCR) assay to measure TL in all sample types for each participant and applied quality control measures to retain only high-quality samples for analysis. We then compared TL from buffy coat and saliva to examine how these measurements differ and to test if TL is correlated across sample types.
resultsTL differed significantly across buffy coat, Oragene saliva, and Oasis saliva samples. TL from buffy coat and Oragene saliva was moderately correlated (ρ = 0.48, P = .002) and the most similar in size. Oasis saliva TL was not correlated with buffy coat or Oragene saliva TL, and was the shortest. DBS DNA yields were inadequate for TL measurement using the MMQPCR assay.
conclusionsUsing a matched dataset we demonstrate that sample type significantly influences the TL measurement obtained using the MMQPCR assay.
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