ArticleThe Indian journal of medical research2017
MALDI-TOF mass spectrometry: An emerging tool for unequivocal identification of non-fermenting Gram-negative bacilli.
Article in The Indian journal of medical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed.
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- Burkholderia cepacia complex nosocomial outbreaks in India: A scoping review.The Indian journal of medical research · 2024Article
- Review
- Molecular epidemiology and molecular typing methods ofSaudi medical journal · 2024Review
- A study on the occurrence of Burkholderia cepacia complex in ultrasound gels used in different veterinary clinical settings in India.Veterinary research communications · 2023Article
- Molecular typing methods & resistance mechanisms of MDRAIMS microbiology · 2023Review
- Multicenter Evaluation of Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) Spectroscopy-Based Method for Rapid Identification of Clinically Relevant Yeasts.Journal of clinical microbiology · 2022Article
- Rapid detection ofFrontiers in cellular and infection microbiology · 2022Article
- Methodological tools to study species of the genus Burkholderia.Applied microbiology and biotechnology · 2021Review
- Advances in the Microbiology of Stenotrophomonas maltophilia.Clinical microbiology reviews · 2021Article
- Evaluation of Dietary Supplements Containing Viable Bacteria by Cultivation/MALDI-TOF Mass Spectrometry and PCR Identification.Frontiers in microbiology · 2021Article
- Antimicrobial susceptibility pattern ofThe Indian journal of medical research · 2020Article
- Matrix-assisted laser desorption/ionization time-of-flight MS for the accurate identification ofJournal of medical microbiology · 2020Article
- Distribution of carbapenemase genes in clinical isolates ofThe Indian journal of medical research · 2020Article
- Quantitative real-time PCR assay for the rapid identification of the intrinsically multidrug-resistant bacterial pathogenMicrobial genomics · 2019Article
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7 authors.
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Abstract
BACKGROUND &
objectivesMatrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been instrumental in revolutionizing microbiological identification, especially in high-throughput laboratories. It has enabled the identification of organisms like non-fermenting Gram-negative bacilli (NFGNB), which has been a challenging task using conventional methods alone. In this study an attempt was made to validate MALDI-TOF MS for the identification of clinical isolates of each of the three most common NFGNB, other than Pseudomonas spp., taking molecular methods as the gold standard.
methodsOne hundred and fifty clinical isolates of NFGNB, confirmed by molecular methods such as Acinetobacter baumannii[oxa-51 polymerase chain reaction (PCR)], Burkholderia cepacia complex (expanded multilocus sequence typing) and Stenotrophomonas maltophilia (species-specific PCR), were taken. Isolated colonies from fresh cultures of all 150 isolates were smeared onto ground steel plate, with and without formic acid extraction step. The identification was carried out using MALDI-TOF MS Biotyper database.
resultsA concordance of 100 and 73.33 per cent was found between the molecular techniques and MALDI-TOF MS system in the identification of these isolates up to genus and species levels, respectively. Using a cut-off of 1.9 for reliable identification, rate of species identification rose to 82.66 per cent. Principal component analysis dendrogram and cluster analysis further increased discrimination of isolates. INTERPRETATION &
conclusionsOur findings showed MALDI-TOF MS-based identification of NFGNB as a good, robust method for high-throughput laboratories.
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