ArticleScientific reports2017
Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers.
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Who cites it
131 citing papers in PubMed.
- Environmental DNA (eDNA) from humans: Review of recent advancements and forensic implications.International journal of legal medicine · 2026Review
- Surveying Biodiverse Coral Reef Ecosystems Across the Tree of Life With Environmental DNA.Molecular ecology resources · 2026Article
- Dive surveys and eDNA detect contrasting macroalgal community composition in Nova Scotia, Canada.Journal of phycology · 2026Article
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- Metagenomic Insights Into Red Sea Biodiversity Across the Web of Life.Environmental microbiology · 2026Article
- eDNA Metabarcoding Reveals Diel Connectivity Dynamics of Fish Communities in Xincun Lagoon, Hainan.Animals : an open access journal from MDPI · 2026Article
- A Roadmap for Using Hybridisation Capture-Based Target Enrichment of Ancient Environmental DNA in Palaeoecology.Molecular ecology resources · 2026Review
- Spatial Complexity and Rare-Taxon Turnover Structures Benthic Coral Reef eDNA Communities.Molecular ecology · 2026Article
- Environmental DNA: From Promise to Practice.Ecology and evolution · 2026Article
- Automated eDNA and eRNA profiling for biodiversity monitoring in marine and freshwater ecosystems.Scientific reports · 2026Article
- Integrating active and passive environmental DNA sampling into coral reef monitoring: implications for community-based stewardship in Hawai i.Research square · 2026Article
- Long-read nanopore shotgun metagenomic DNA sequencing for river biodiversity, wildlife, pollution, and environmental health monitoring.NAR genomics and bioinformatics · 2026Article
- Aligning observation to action: Integrated monitoring for tropical seascape conservation and restoration.iScience · 2026Review
- Evaluating the Molecular Potential and Interpretability of DNA in Historical Spirit Collection Media.Molecular ecology resources · 2026Article
- Environmental DNA reveals hidden eukaryotic diversity and fine-scale community patterns across seascape areas in the Northern Red Sea.Scientific reports · 2026Article
- Validating COI eDNA Metabarcoding Primers for Detection of Subterranean Fauna.Molecular ecology resources · 2026Article
- Optimizing a Novel eDNA-Based Framework for Reef Fish Biodiversity Monitoring Using an Autonomous Filtration System and in situ Nanopore Sequencing.Ecology and evolution · 2026Article
- A tailored MoSNational science review · 2026Article
- Residual eDNA in eRNA Extracts Skews eRNA-Based Biodiversity Assessment: Call for Optimised DNase Treatment.Molecular ecology resources · 2026Article
- Diet and gut microbiome of skipjack tuna (Katsuwonus pelamis) as indicators of environmental changes.PloS one · 2026Article
71 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effective marine management requires comprehensive data on the status of marine biodiversity. However, efficient methods that can document biodiversity in our oceans are currently lacking. Environmental DNA (eDNA) sourced from seawater offers a new avenue for investigating the biota in marine ecosystems. Here, we investigated the potential of eDNA to inform on the breadth of biodiversity present in a tropical marine environment. Directly sequencing eDNA from seawater using a shotgun approach resulted in only 0.34% of 22.3 million reads assigning to eukaryotes, highlighting the inefficiency of this method for assessing eukaryotic diversity. In contrast, using 'tree of life' (ToL) metabarcoding and 20-fold fewer sequencing reads, we could detect 287 families across the major divisions of eukaryotes. Our data also show that the best performing 'universal' PCR assay recovered only 44% of the eukaryotes identified across all assays, highlighting the need for multiple metabarcoding assays to catalogue biodiversity. Lastly, focusing on the fish genus Lethrinus, we recovered intra- and inter-specific haplotypes from seawater samples, illustrating that eDNA can be used to explore diversity beyond taxon identifications. Given the sensitivity and low cost of eDNA metabarcoding we advocate this approach be rapidly integrated into biomonitoring programs.
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