ArticleScientific reports2017
ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Stem Cell-Derived Embryo Models: Potential Platforms for Investigating Early Development and Embryotoxicity.International journal of stem cells · 2025Review
- Live imaging endogenous transcription factor dynamics reveals mechanisms of epiblast and primitive endoderm fate segregation.Current biology : CB · 2025Article
- Advances in the Study of Pluripotent Stem Cells in Livestock.Cell proliferation · 2025Review
- AI-powered simulation-based inference of a genuinely spatial-stochastic gene regulation model of early mouse embryogenesis.PLoS computational biology · 2024Article
- A geometrical model of cell fate specification in the mouse blastocyst.Development (Cambridge, England) · 2024Article
- Paracrine interactions through FGFR1 and FGFR2 receptors regulate the development of preimplantation mouse chimaeric embryo.Open biology · 2022Article
- H3K9 tri-methylation at Nanog times differentiation commitment and enables the acquisition of primitive endoderm fate.Development (Cambridge, England) · 2022Article
- NANOG initiates epiblast fate through the coordination of pluripotency genes expression.Nature communications · 2022Article
- Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos.Scientific reports · 2021Article
- DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification.Open biology · 2021Article
- p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice.Communications biology · 2021Article
- A multiscale model via single-cell transcriptomics reveals robust patterning mechanisms during early mammalian embryo development.PLoS computational biology · 2021Article
- Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development.Frontiers in cell and developmental biology · 2021Review
- Live Visualization of ERK Activity in the Mouse Blastocyst Reveals Lineage-Specific Signaling Dynamics.Developmental cell · 2020Article
- Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo.Developmental cell · 2020Article
- Coordination between patterning and morphogenesis ensures robustness during mouse development.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Mouse ICM Organoids Reveal Three-Dimensional Cell Fate Clustering.Biophysical journal · 2019Article
- The Pleiotropic Effects of the Canonical Wnt Pathway in Early Development and Pluripotency.Genes · 2018Review
- Plasticity of the inner cell mass in mouse blastocyst is restricted by the activity of FGF/MAPK pathway.Scientific reports · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inner cell Mass (ICM) specification into epiblast (Epi) and primitive endoderm (PrE) is an asynchronous and progressive process taking place between E3.0 to E3.75 under the control of the Fibroblast Growth Factor (FGF)/Extracellular signal-Regulated Kinase (ERK) signaling pathway. Here, we have analyzed in details the kinetics of specification and found that ICM cell responsiveness to the up and down regulation of FGF signaling activity are temporally distinct. We also showed that PrE progenitors are generated later than Epi progenitors. We further demonstrated that, during this late phase of specification, a 4 hours period of FGF/ERK inhibition prior E3.75 is sufficient to convert ICM cells into Epi. Finally, we showed that ICM conversion into Epi in response to inhibition during this short time window requires both transcription and proteasome degradation. Collectively, our data give new insights into the timing and mechanisms involved in the process of ICM specification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.