ArticleBMC veterinary research2017
Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Genomic Characterization and Pathogenicity Island Analysis of 17 Mexican Isolates ofCurrent issues in molecular biology · 2026Article
- Development and Validation of a High-Resolution Melting (HRM) Method for Differentiating Ovis and Equi Biovars ofVeterinary sciences · 2026Article
- Isolation, whole genome sequencing, and comparative genomics of Corynebacterium pseudotuberculosis to identify biovar ovis specific biomarkers.Molecular biology reports · 2026Article
- Genomic diversity and host-specificity inFrontiers in microbiology · 2026Article
- Oral fluid as a material for serological diagnostics of caseous lymphadenitis in goats.Scientific reports · 2025Article
- A snapshot on molecular technologies for diagnosing FAdV infections.Frontiers in veterinary science · 2025Review
- Controlling drug-resistant bacteria in Arabian horses: bacteriophage cocktails for treating wound infections.Frontiers in veterinary science · 2025Article
- Longitudinal Follow-Up of Clinical Superficial Ovine Caseous Lymphadenitis.Animals : an open access journal from MDPI · 2024Article
- Rearing Management and Its Impact on Caseous Lymphadenitis in Sheep.Animals : an open access journal from MDPI · 2024Article
- PCR in Forensic Science: A Critical Review.Genes · 2024Review
- Corynebacterium pseudotuberculosis: Whole genome sequencing reveals unforeseen and relevant genetic diversity in this pathogen.PloS one · 2024Article
- Article
- Simultaneous and rapid detection of avian respiratory diseases of small poultry using multiplex reverse transcription-Polymerase Chain Reaction assay.Poultry science · 2023Article
- Isolation, characterization, and pathogenicity assessment ofFrontiers in microbiology · 2023Article
- Article
- Molecular Characterization ofVeterinary sciences · 2021Article
- Atypical Multibacterial Granulomatous Myositis in a Horse: First Report in Italy.Veterinary sciences · 2020Article
- Article
- Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks.BMC veterinary research · 2019Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCorynebacterium pseudotuberculosis is classified into two biovars, nitrate-negative biovar Ovis which is the etiologic agent of caseous lymphadenitis in small ruminants and nitrate-positive biovar Equi, which causes abscesses and ulcerative lymphangitis in equines. The aim of this study was to develop a quadruplex PCR assay that would allow simultaneous detection and biovar-typing of C. pseudotuberculosis.
methodsIn the present study, genomes of C. pseudotuberculosis strains were used to identify the genes involved in the nitrate reduction pathway to improve a species identification three-primer multiplex PCR assay. The nitrate reductase gene (narG) was included in the PCR assay along with the 16S, rpoB and pld genes to enhance the diagnosis of the multiplex PCR at biovar level.
resultsA novel quadruplex PCR assay for C. pseudotuberculosis species and biovar identification was developed. The results of the quadruplex PCR of 348 strains, 346 previously well-characterized clinical isolates of C. pseudotuberculosis from different hosts (goats, sheep, horse, cattle, buffalo, llamas and humans), the vaccine strain 1002 and the type strain ATCC 19410
conclusionsThe ability of the quadruplex assay to discriminate between C. pseudotuberculosis biovar Ovis and Equi strains enhances its usefulness in the clinical microbiology laboratory.
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