ArticlePloS one2017
The association between DNA methylation and exon expression in the Pacific oyster Crassostrea gigas.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- BDNF-DT and BDNF-AS-DT: novel genes in the BDNF locus.Molecular psychiatry · 2026Article
- Integration of epigenetics into ecotoxicology: insights and fundamental research needs.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Article
- DNA methylation correlates with transcriptional noise in response to elevated pCOEnvironmental epigenetics · 2024Article
- The methylome and cell-free DNA: current applications in medicine and pediatric disease.Pediatric research · 2023Review
- Associations between DNA methylation and gene regulation depend on chromatin accessibility during transgenerational plasticity.BMC biology · 2023Article
- Epigenetic and Genetic Population Structure is Coupled in a Marine Invertebrate.Genome biology and evolution · 2023Article
- Comparative Methylome Analysis Reveals Epigenetic Signatures Associated with Growth and Shell Color in the Pacific Oyster, Crassostrea gigas.Marine biotechnology (New York, N.Y.) · 2022Article
- Epigenetic machinery is functionally conserved in cephalopods.BMC biology · 2022Article
- Differential DNA methylation in Pacific oyster reproductive tissue in response to ocean acidification.BMC genomics · 2022Article
- Review
- Splicing alterations in healthy aging and disease.Wiley interdisciplinary reviews. RNA · 2021Review
- Inference of DNA methylation patterns in molluscs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis.International journal of molecular sciences · 2020Article
- Article
- Characterization of Global DNA Methylation in Different Gene Regions Reveals Candidate Biomarkers in Pigs with High and Low Levels of Boar Taint.Veterinary sciences · 2020Article
- Population epigenetic divergence exceeds genetic divergence in the Eastern oysterEvolutionary applications · 2020Article
- Methylation of the first exon in the erythropoietin receptor gene does not correlate with its mRNA and protein level in cancer cells.BMC genetics · 2019Article
- The importance of DNA methylation of exons on alternative splicing.RNA (New York, N.Y.) · 2018Article
- Pattern of DNA Methylation in Daphnia: Evolutionary Perspective.Genome biology and evolution · 2018Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA methylation is one of the most important epigenetic modifications of eukaryotic genomes and is believed to play integral roles in diverse biological processes. Although DNA methylation has been well studied in mammals, data are limited in invertebrates, particularly Mollusca. The Pacific oyster Crassostrea gigas is an emerging genetic model for functional analysis of DNA methylation in Mollusca. Recent studies have shown that there is a positive association between methylation status and gene expression in C. gigas; however, whether this association exists at the exon level remains to be determined.
resultsIn this study, we characterized the genome-wide methylation pattern across two different tissues of C. gigas and found that methylated genes are expressed in more tissues and development stages than unmethylated genes. Furthermore, we found that different types of exons had different methylation levels, with the lowest methylation levels in the first exons, followed by the last exons, and the internal exons. We found that the exons included in the gene transcript contained significantly higher DNA methylation levels than skipped exons. We observed that the DNA methylation levels increased slowly after the start sites and end sites of exons seperately, and then decreased quickly towards the middle sites of exons. We also found that methylated exons were significantly longer than unmethylated exons.
conclusionThis study constitutes the first genome-wide analysis to show an association between exon-level DNA methylation and mRNA expression in the oyster. Our findings suggest that exon-level DNA methylation may play a role in the construction of alternative splicing by positively influencing exon inclusion during transcription.
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