ArticleAnalytical chemistry2017
Integration of Ion Mobility MS
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Thermal Denaturation of Fresh Frozen Tissue Enhances Mass Spectrometry Detection of Peptides.Analytical chemistry · 2024Article
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Mass spectrometry imaging: new eyes on natural products for drug research and development.Acta pharmacologica Sinica · 2022Review
- Quantitative mass spectrometry imaging of drugs and metabolites: a multiplatform comparison.Analytical and bioanalytical chemistry · 2021Article
- Spatially resolved absolute quantitation in thin tissue by mass spectrometry.Analytical and bioanalytical chemistry · 2021Review
- Single-Cell Metabolomics by Mass Spectrometry Imaging.Advances in experimental medicine and biology · 2021Article
- Neuropeptidomics: Improvements in Mass Spectrometry Imaging Analysis and Recent Advancements.Current protein & peptide science · 2021Review
- Nano-liquid chromatography-mass spectrometry and recent applications in omics investigations.Analytical methods : advancing methods and applications · 2020Review
- Cellular resolution in clinical MALDI mass spectrometry imaging: the latest advancements and current challenges.Clinical chemistry and laboratory medicine · 2020Review
- LESA Cyclic Ion Mobility Mass Spectrometry of Intact Proteins from Thin Tissue Sections.Analytical chemistry · 2020Article
- Localization of sterols and oxysterols in mouse brain reveals distinct spatial cholesterol metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates.Biochemical Society transactions · 2020Review
- Probing the Fundamentals of Native Liquid Extraction Surface Analysis Mass Spectrometry of Proteins: Can Proteins Refold during Extraction?Analytical chemistry · 2019Article
- MicroLESA: Integrating Autofluorescence Microscopy, In Situ Micro-Digestions, and Liquid Extraction Surface Analysis for High Spatial Resolution Targeted Proteomic Studies.Analytical chemistry · 2019Article
- Protein identification strategies in MALDI imaging mass spectrometry: a brief review.Current opinion in chemical biology · 2019Review
- Imaging Isomers on a Biological Surface: A Review.Mass spectrometry (Tokyo, Japan) · 2019Article
- Targeted Drug and Metabolite Imaging: Desorption Electrospray Ionization Combined with Triple Quadrupole Mass Spectrometry.Analytical chemistry · 2018Article
- Ambient ionisation mass spectrometry for in situ analysis of intact proteins.Journal of mass spectrometry : JMS · 2018Article
- Protein identification in imaging mass spectrometry through spatially targeted liquid micro-extractions.Rapid communications in mass spectrometry : RCM · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct analysis by mass spectrometry (imaging) has become increasingly deployed in preclinical and clinical research due to its rapid and accurate readouts. However, when it comes to biomarker discovery or histopathological diagnostics, more sensitive and in-depth profiling from localized areas is required. We developed a comprehensive, fully automated online platform for high-resolution liquid extraction surface analysis (HR-LESA) followed by micro-liquid chromatography (LC) separation and a data-independent acquisition strategy for untargeted and low abundant analyte identification directly from tissue sections. Applied to tissue sections of rat pituitary, the platform demonstrated improved spatial resolution, allowing sample areas as small as 400 μm to be studied, a major advantage over conventional LESA. The platform integrates an online buffer exchange and washing step for removal of salts and other endogenous contamination that originates from local tissue extraction. Our carry over-free platform showed high reproducibility, with an interextraction variability below 30%. Another strength of the platform is the additional selectivity provided by a postsampling gas-phase ion mobility separation. This allowed distinguishing coeluted isobaric compounds without requiring additional separation time. Furthermore, we identified untargeted and low-abundance analytes, including neuropeptides deriving from the pro-opiomelanocortin precursor protein and localized a specific area of the pituitary gland (i.e., adenohypophysis) known to secrete neuropeptides and other small metabolites related to development, growth, and metabolism. This platform can thus be applied for the in-depth study of small samples of complex tissues with histologic features of ∼400 μm or more, including potential neuropeptide markers involved in many diseases such as neurodegenerative diseases, obesity, bulimia, and anorexia nervosa.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.