ArticleNature nanotechnology2017
Focal molography is a new method for the in situ analysis of molecular interactions in biological samples.
Article in Nature nanotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
- Organization and Dynamics of Focal Adhesions: Light Diffraction Analysis of Cellular Adhesion on Nanopatterned Surfaces.ACS applied materials & interfaces · 2026Article
- Investigations into linker effects of DNA-VHL ligand conjugates by multiplexed affinity measurements using focal molography.RSC chemical biology · 2026Article
- Optical Waveguide Lightmode Spectroscopy: A Versatile Technique for Real-Time, Label-Free Biosensing.Sensors (Basel, Switzerland) · 2026Review
- High-throughput thermodynamic fingerprinting of protein-ligand interactions by DNA-directed focal molography.PloS one · 2026Article
- Article
- Focal Molography Allows for Affinity and Concentration Measurements of Proteins in Complex Matrices with High Accuracy.Biosensors · 2025Article
- Exploring β-catenin and TCF4 interaction in complex environments by means of novel biosensing platform focal molography.PloS one · 2025Article
- Imaging Diffractometric Biosensors for Label-Free, Multi-Molecular Interaction Analysis.Biosensors · 2024Article
- Surface Bragg gratings of proteins patterned on integrated waveguides for (bio)chemical analysis.Mikrochimica acta · 2023Article
- Biomarkers as Biomedical Bioindicators: Approaches and Techniques for the Detection, Analysis, and Validation of Novel Biomarkers of Diseases.Pharmaceutics · 2023Review
- Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy.Biosensors · 2023Article
- Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing.ACS applied materials & interfaces · 2022Article
- Time-resolved fluorescence determination of albumin using ZnGeO:Mn luminescence nanorods modified with polydopamine nanoparticles.Mikrochimica acta · 2021Article
- Ultra-Stable Molecular Sensors by Sub-Micron Referencing and Why They Should Be Interrogated by Optical Diffraction-Part I. The Concept of a Spatial Affinity Lock-in Amplifier.Sensors (Basel, Switzerland) · 2021Article
- Ultra Stable Molecular Sensors by Submicron Referencing and Why They Should Be Interrogated by Optical Diffraction-Part II. Experimental Demonstration.Sensors (Basel, Switzerland) · 2020Article
- Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects.Analytical and bioanalytical chemistry · 2020Review
- Localized detection of ions and biomolecules with a force-controlled scanning nanopore microscope.Nature nanotechnology · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Focal molography is a next-generation biosensor that visualizes specific biomolecular interactions in real time. It transduces affinity modulation on the sensor surface into refractive index modulation caused by target molecules that are bound to a precisely assembled nanopattern of molecular recognition sites, termed the 'mologram'. The mologram is designed so that laser light is scattered at specifically bound molecules, generating a strong signal in the focus of the mologram via constructive interference, while scattering at nonspecifically bound molecules does not contribute to the effect. We present the realization of molograms on a chip by submicrometre near-field reactive immersion lithography on a light-sensitive monolithic graft copolymer layer. We demonstrate the selective and sensitive detection of biomolecules, which bind to the recognition sites of the mologram in various complex biological samples. This allows the label-free analysis of non-covalent interactions in complex biological samples, without a need for extensive sample preparation, and enables novel time- and cost-saving ways of performing and developing immunoassays for diagnostic tests.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.