ArticleeLife2017
A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Circuit-constrained molecular neuroethology in the medicinal leech Hirudo verbana.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- Age-related degradation of behavioral and network features ofFrontiers in aging neuroscience · 2026Article
- Age-related degradation of behavioral and network features ofbioRxiv : the preprint server for biology · 2025Article
- Kilohertz volumetric imaging of in vivo dynamics using squeezed light field microscopy.Nature methods · 2025Article
- Current Practice in Using Voltage Imaging to Record Fast Neuronal Activity: Successful Examples from Invertebrate to Mammalian Studies.Biosensors · 2023Review
- Cell anatomy and network input explain differences within but not between leech touch cells at two different locations.Frontiers in cellular neuroscience · 2023Article
- Voltage imaging identifies spinal circuits that modulate locomotor adaptation in zebrafish.Neuron · 2022Article
- Initial Variability and Time-Dependent Changes of Neuronal Response Features Are Cell-Type-Specific.Frontiers in cellular neuroscience · 2022Article
- Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits.eLife · 2021Article
- Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates.Journal of visualized experiments : JoVE · 2020Article
- Electrophysiology, Unplugged: Imaging Membrane Potential with Fluorescent Indicators.Accounts of chemical research · 2020Review
- Monitoring neuronal activity with voltage-sensitive fluorophores.Methods in enzymology · 2020Article
- Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia.Bio-protocol · 2018Article
- Effects of Touch Location and Intensity on Interneurons of the Leech Local Bend Network.Scientific reports · 2018Article
- Higher Network Activity Induced by Tactile Compared to Electrical Stimulation of Leech Mechanoreceptors.Frontiers in physiology · 2018Article
- A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech.eLife · 2017Article
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Authors and funding
2 authors.
Funding
Abstract
Studies of neuronal network emergence during sensory processing and motor control are greatly facilitated by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging, we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.
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