ArticleOptica2017
Imaging Subcellular Dynamics with Fast and Light-Efficient Volumetrically Parallelized Microscopy.
Article in Optica, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Volumetric imaging and computation to explore contractile function in zebrafish hearts.Cell reports methods · 2025Article
- Compact, high-speed multi-directional selective plane illumination microscopy.Biomedical optics express · 2023Article
- Axial scanning of dual focus to improve light sheet microscopy.Biomedical optics express · 2022Article
- Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array.Laser & photonics reviews · 2022Article
- KHz-rate volumetric voltage imaging of the whole Zebrafish heart.Biophysical reports · 2022Article
- Real-time multi-angle projection imaging of biological dynamics.Nature methods · 2021Article
- Fast, multiplane line-scan confocal microscopy using axially distributed slits.Biomedical optics express · 2021Article
- DMD-based hyperspectral microscopy with flexible multiline parallel scanning.Microsystems & nanoengineering · 2021Article
- The low-cost Shifter microscope stage transforms the speed and robustness of protein crystal harvesting.Acta crystallographica. Section D, Structural biology · 2021Article
- A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics.eLife · 2020Article
- Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array.Light, science & applications · 2020Article
- Fast objective coupled planar illumination microscopy.Nature communications · 2019Article
- Mutant p53 amplifies a dynamin-1/APPL1 endosome feedback loop that regulates recycling and migration.The Journal of cell biology · 2019Article
- Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution.Nature methods · 2019Article
- Fast, volumetric live-cell imaging using high-resolution light-field microscopy.Biomedical optics express · 2019Article
- Sensitive light-sheet microscopy in multiwell plates using an AFM cantilever.Biomedical optics express · 2018Article
- Reversal of ApoE4-induced recycling block as a novel prevention approach for Alzheimer's disease.eLife · 2018Article
- Advances in Imaging Plant Cell Dynamics.Plant physiology · 2018Review
- Lossless Three-Dimensional Parallelization in Digitally Scanned Light-Sheet Fluorescence Microscopy.Scientific reports · 2017Article
- Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens.Nature biomedical engineering · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
In fluorescence microscopy, the serial acquisition of 2D images to form a 3D volume limits the maximum imaging speed. This is particularly evident when imaging adherent cells in a light-sheet fluorescence microscopy format, as their elongated morphologies require ~200 image planes per image volume. Here, by illuminating the specimen with three light-sheets, each independently detected, we present a light-efficient, crosstalk free, and volumetrically parallelized 3D microscopy technique that is optimized for high-speed (up to 14 Hz) subcellular (300 nm lateral, 600 nm axial resolution) imaging of adherent cells. We demonstrate 3D imaging of intracellular processes, including cytoskeletal dynamics in single cell migration and collective wound healing for 1500 and 1000 time points, respectively. Further, we capture rapid biological processes, including trafficking of early endosomes with velocities exceeding 10 microns per second and calcium signaling in primary neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.