ArticleBone2017
Ex vivo construction of human primary 3D-networked osteocytes.
Article in Bone, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Harnessing Advances in Bone Tissue Engineering for Design of Bone-on-Chip Systems.Advanced healthcare materials · 2026Review
- Rethinking osteocyte modelsFrontiers in bioengineering and biotechnology · 2026Review
- Autonomous Implants.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- 3D Osteocyte Networks under Pulsatile Unidirectional Fluid Flow Stimuli (PUFFS).ACS biomaterials science & engineering · 2025Article
- Development of a BMU-on-a-chip model based on spatiotemporal regulation of cellular interactions in the bone remodeling cycle.Materials today. Bio · 2025Article
- Cellnet technology to generate 3D, functional, single-cell networks in custom architectures within collagen.Biofabrication · 2025Article
- Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model.JBMR plus · 2023Article
- Article
- Osteon: Structure, Turnover, and Regeneration.Tissue engineering. Part B, Reviews · 2022Review
- A Three-Dimensional Mechanical Loading Model of Human Osteocytes in Their Native Matrix.Calcified tissue international · 2022Article
- Natural killer cells activity against multiple myeloma cells is modulated by osteoblast-induced IL-6 and IL-10 production.Heliyon · 2022Article
- A Bioreactor for 3DFrontiers in bioengineering and biotechnology · 2022Article
- Triple Culture of Primary Human Osteoblasts, Osteoclasts and Osteocytes as an In Vitro Bone Model.International journal of molecular sciences · 2021Article
- Organ-on-a-chip platforms for accelerating the evaluation of nanomedicine.Bioactive materials · 2021Review
- Cell Sources for Human In vitro Bone Models.Current osteoporosis reports · 2021Review
- New and Old Osteocytic Cell Lines and 3D Models.Current osteoporosis reports · 2020Review
- Primary Human Osteoblasts Cultured in a 3D Microenvironment Create a Unique Representative Model of Their Differentiation Into Osteocytes.Frontiers in bioengineering and biotechnology · 2020Article
- Investigating Osteocytic Perilacunar/Canalicular Remodeling.Current osteoporosis reports · 2019Review
- Studies on Osteocytes in Their 3D Native Matrix Versus 2D In Vitro Models.Current osteoporosis reports · 2019Review
- Human ex vivo 3D bone model recapitulates osteocyte response to metastatic prostate cancer.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A human bone tissue model was developed by constructing ex vivo the 3D network of osteocytes via the biomimetic assembly of primary human osteoblastic cells with 20-25μm microbeads and subsequent microfluidic perfusion culture. The biomimetic assembly: (1) enabled 3D-constructed cells to form cellular network via processes with an average cell-to-cell distance of 20-25μm, and (2) inhibited cell proliferation within the interstitial confine between the microbeads while the confined cells produced extracellular matrix (ECM) to form a mechanically integrated structure. The mature osteocytic expressions of SOST and FGF23 genes became significantly higher, especially for SOST by 250 folds during 3D culture. The results validate that the bone tissue model: (1) consists of 3D cellular network of primary human osteocytes, (2) mitigates the osteoblastic differentiation and proliferation of primary osteoblast-like cells encountered in 2D culture, and (3) therefore reproduces ex vivo the phenotype of human 3D-networked osteocytes. The 3D tissue construction approach is expected to provide a clinically relevant and high-throughput means for evaluating drugs and treatments that target bone diseases with in vitro convenience.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.