ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2017
Imaging flow cytometry assays for quantifying pigment grade titanium dioxide particle internalization and interactions with immune cells in whole blood.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- CD71Scandinavian journal of immunology · 2026Article
- Identifying vascular stiffening-sensitive macrophages through integration of single-cell transcriptomics and imaging flow cytometry.Biophysics reports · 2025Article
- Multiscale Profiling of Nanoscale Metal-Organic Framework Biocompatibility and Immune Interactions.Advanced healthcare materials · 2025Article
- Human Primary Monocytes as a Model for in vitro Immunotoxicity Testing: Evaluation of the Regulatory Properties of TiOInternational journal of nanomedicine · 2025Article
- Distinct targeting and uptake of platelet and red blood cell-derived extracellular vesicles into immune cells.Journal of extracellular biology · 2024Article
- Label-Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Gastrointestinal Absorption and Toxicity of Nanoparticles and Microparticles: Myth, Reality and Pitfalls explored through Titanium Dioxide.Current opinion in toxicology · 2020Article
- Ultrasmall silica nanoparticles directly ligate the T cell receptor complex.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Imaging flow cytometry methods for quantitative analysis of label-free crystalline silica particle interactions with immune cells.AIMS biophysics · 2020Article
- In Vitro Methods for Assessing Nanoparticle Toxicity.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing.Particle and fibre toxicology · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Pigment grade titanium dioxide is composed of sub-micron sized particles, including a nanofraction, and is widely utilized in food, cosmetic, pharmaceutical, and biomedical industries. Oral exposure to pigment grade titanium dioxide results in at least some material entering the circulation in humans, although subsequent interactions with blood immune cells are unknown. Pigment grade titanium dioxide is employed for its strong light scattering properties, and this work exploited that attribute to determine whether single cell-particle associations could be determined in immune cells of human whole blood at "real life" concentrations. In vitro assays, initially using isolated peripheral blood mononuclear cells, identified titanium dioxide associated with the surface of, and within, immune cells by darkfield reflectance in imaging flow cytometry. This was confirmed at the population level by side scatter measurements using conventional flow cytometry. Next, it was demonstrated that imaging flow cytometry could quantify titanium dioxide particle-bearing cells, within the immune cell populations of fresh whole blood, down to titanium dioxide levels of 10 parts per billion, which is in the range anticipated for human blood following titanium dioxide ingestion. Moreover, surface association and internal localization of titanium dioxide particles could be discriminated in the assays. Overall, results showed that in addition to the anticipated activity of blood monocytes internalizing titanium dioxide particles, neutrophil internalization and cell membrane adhesion also occurred, the latter for both phagocytic and nonphagocytic cell types. What happens in vivo and whether this contributes to activation of one or more of these different cells types in blood merits further attention. © 2017 International Society for Advancement of Cytometry.
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