Evidence map›Paper›PMID 28939853›Full record

ArticleNature communications2017

RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane.

Daryll Vanover, Daisy V Smith, Emmeline L Blanchard, Eric Alonas, Jonathan L Kirschman, Aaron W Lifland, Chiara Zurla, Philip J Santangelo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 43 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Introduction to Respiratory Syncytial Virus.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  6. Review
  7. Host Subcellular Organelles: Targets of Viral Manipulation.International journal of molecular sciences · 2024
    Review
  8. Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Viral NNature communications · 2019
    Article
  18. Article
  19. Respiratory syncytial virus entry and how to block it.Nature reviews. Microbiology · 2019
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Daryll VanoverWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, 30332, USA.
Daisy V SmithWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, 30332, USA.
Emmeline L BlanchardWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, 30332, USA.
Eric AlonasWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, 30332, USA.
Jonathan L KirschmanWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, 30332, USA.
Aaron W LiflandInstitute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, 30332, USA.
Chiara ZurlaWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, 30332, USA.
Philip J SantangeloWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, 30332, USA. philip.santangelo@bme.gatech.edu.
The Wallace H. Coulter Department of Biomedical Engineering · USGeorgia Institute of Technology · US

Funding

Single RNA sensitive probes for studying viral replication and buddingR01GM094198 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI SANTANGELO, PHILIP J · 2010 to 2014
$2.0M
Structural Investigations Of Macromolecular Complexes Critical To hRSV Life CycleR01GM114561 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SANTANGELO, PHILIP J, WRIGHT, ELIZABETH R · 2016 to 2019
$1.6M
NIGMS NIH HHS R01 GM094198NIGMS NIH HHS R01 GM114561
6 · The paper itself

Abstract

The human respiratory syncytial virus G protein plays an important role in the entry and assembly of filamentous virions. Here, we report the use of fluorescently labeled soybean agglutinin to selectively label the respiratory syncytial virus G protein in living cells without disrupting respiratory syncytial virus infectivity or filament formation and allowing for interrogations of respiratory syncytial virus virion assembly. Using this approach, we discovered that plasma membrane-bound respiratory syncytial virus G rapidly recycles from the membrane via clathrin-mediated endocytosis. This event is then followed by the dynamic formation of filamentous and branched respiratory syncytial virus particles, and assembly with genomic ribonucleoproteins and caveolae-associated vesicles prior to re-insertion into the plasma membrane. We demonstrate that these processes are halted by the disruption of microtubules and inhibition of molecular motors. Collectively, our results show that for respiratory syncytial virus assembly, viral filaments are produced and loaded with genomic RNA prior to insertion into the plasma membrane.Assembly of filamentous RSV particles is incompletely understood due to a lack of techniques suitable for live-cell imaging. Here Vanover et al. use labeled soybean agglutinin to selectively label RSV G protein and show how filamentous RSV assembly, initiated in the cytoplasm, uses G protein recycled from the plasma membrane.

Indexed as

AnimalsCaveolinsCell MembraneChlorocebus aethiopsClathrinEndocytosisHumansMicrotubulesMolecular ImagingPhytohemagglutininsPlant LectinsRespiratory Syncytial Virus, HumanRibonucleoproteinsRNA, ViralSoybean ProteinsVero CellsCaveolinsClathrinG glycoprotein, Respiratory syncytial virusPhytohemagglutininsPlant LectinsRibonucleoproteinsRNA, Viralsoybean lectinSoybean ProteinsViral Fusion Proteins

Identifiers

PMID28939853
PMCPMC5610308
OpenAlexW2755941298

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.