ArticleRNA (New York, N.Y.)2017
Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI.
Article in RNA (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Bright and pH-sensitive Baby Spinach aptamer with RNA triplex fusion.Nucleic acids research · 2025Article
- Elucidating the solution structure of the monomolecularChemical science · 2025Article
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- RNA tertiary structure and conformational dynamics revealed by BASH MaP.bioRxiv : the preprint server for biology · 2024Article
- Structure-based insights into fluorogenic RNA aptamers.Acta biochimica et biophysica Sinica · 2024Review
- Fluorogenic RNA-Based Biosensors of Small Molecules: Current Developments, Uses, and Perspectives.Biosensors · 2024Review
- Structural mechanisms for binding and activation of a contact-quenched fluorophore by RhoBAST.Nature communications · 2024Article
- Comprehensive analysis of intramolecular G-quadruplex structures: furthering the understanding of their formalism.Nucleic acids research · 2024Article
- Resolving a guanine-quadruplex structure in the SARS-CoV-2 genome through circular dichroism and multiscale molecular modeling.Chemical science · 2023Article
- RNA structure determination: From 2D to 3D.Fundamental research · 2023Review
- Intricate 3D architecture of a DNA mimic of GFP.Nature · 2023Article
- RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli-Pepper aptamer FRET pair.Nucleic acids research · 2023Article
- ASC-G4, an algorithm to calculate advanced structural characteristics of G-quadruplexes.Nucleic acids research · 2023Article
- The Functional Meaning of 5'UTR in Protein-Coding Genes.International journal of molecular sciences · 2023Review
- A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer.Sensors and actuators reports · 2022Article
- High-throughput iSpinach fluorescent aptamer-based real-time monitoring of in vitro transcription.Bioresources and bioprocessing · 2022Article
- Ligands with polyfluorophenyl moieties promote a local structural rearrangement in the Spinach2 and Broccoli aptamers that increases ligand affinities.RNA (New York, N.Y.) · 2022Article
- The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold.Nature chemical biology · 2022Article
- RNA sequence and structure control assembly and function of RNA condensates.RNA (New York, N.Y.) · 2021Article
- Transcription complexes as RNA chaperones.Transcription · 2021Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorogenic RNA aptamers are short nucleic acids able to specifically interact with small molecules and strongly enhance their fluorescence upon complex formation. Among the different systems recently introduced, Spinach, an aptamer forming a fluorescent complex with the 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI), is one of the most promising. Using random mutagenesis and ultrahigh-throughput screening, we recently developed iSpinach, an improved version of the aptamer, endowed with an increased folding efficiency and thermal stability. iSpinach is a shorter version of Spinach, comprising five mutations for which the exact role has not yet been deciphered. In this work, we cocrystallized a reengineered version of iSpinach in complex with the DFHBI and solved the X-ray structure of the complex at 2 Å resolution. Only a few mutations were required to optimize iSpinach production and crystallization, underlying the good folding capacity of the molecule. The measured fluorescence half-lives in the crystal were 60% higher than in solution. Comparisons with structures previously reported for Spinach sheds some light on the possible function of the different beneficial mutations carried by iSpinach.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.