ArticleAmerican journal of physiology. Heart and circulatory physiology2017
Intracardiac light catheter for rapid scanning transmural absorbance spectroscopy of perfused myocardium: measurement of myoglobin oxygenation and mitochondria redox state.
Article in American journal of physiology. Heart and circulatory physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 17 citations in OpenAlex.
- Panoramic hyperspectral optical mapping of cardiac membrane potential and tissue type.Journal of biomedical optics · 2026Article
- Rapid mitochondrial repolarization upon reperfusion after cardiac ischemia.Nature cardiovascular research · 2025Article
- Effect of the mitochondrial membrane potential on the absorbance of the reduced form of cytochrome c oxidase.Biochimica et biophysica acta. Bioenergetics · 2024Article
- Oxidation-reduction imaging of myoglobin reveals two-phase oxidation in the reperfused myocardium.Basic research in cardiology · 2024Article
- Mechanisms of reduced myocardial energetics of the dystrophic heart.American journal of physiology. Heart and circulatory physiology · 2024Article
- Pyruvate modulation of redox potential controls mouse sperm motility.Developmental cell · 2024Article
- Cardiac nitric oxide scavenging: role of myoglobin and mitochondria.The Journal of physiology · 2024Article
- Increased mitochondrial free CaCell reports · 2023Article
- Spectroscopic identification of the catalytic intermediates of cytochrome c oxidase in respiring heart mitochondria.Biochimica et biophysica acta. Bioenergetics · 2023Article
- Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart.Cell reports · 2021Article
- Energy homeostasis is a conserved process: Evidence from Paracoccus denitrificans' response to acute changes in energy demand.PloS one · 2021Article
- Mitochondria and the thermal limits of ectotherms.The Journal of experimental biology · 2020Review
- Perfused murine heart optical transmission spectroscopy using optical catheter and integrating sphere: Effects of ischemia/reperfusion.Analytical biochemistry · 2019Article
- Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts.Journal of visualized experiments : JoVE · 2019Article
- Paradoxical arteriole constriction compromises cytosolic and mitochondrial oxygen delivery in the isolated saline-perfused heart.American journal of physiology. Heart and circulatory physiology · 2018Article
- Cardiac performance is limited by oxygen delivery to the mitochondria in the crystalloid-perfused working heart.American journal of physiology. Heart and circulatory physiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Absorbance spectroscopy of intrinsic cardiac chromophores provides nondestructive assessment of cytosolic oxygenation and mitochondria redox state. Isolated perfused heart spectroscopy is usually conducted by collecting reflected light from the heart surface, which represents a combination of surface scattering events and light that traversed portions of the myocardium. Reflectance spectroscopy with complex surface scattering effects in the beating heart leads to difficulty in quantitating chromophore absorbance. In this study, surface scattering was minimized and transmural path length optimized by placing a light source within the left ventricular chamber while monitoring transmurally transmitted light at the epicardial surface. The custom-designed intrachamber light catheter was a flexible coaxial cable (2.42-Fr) terminated with an encapsulated side-firing LED of 1.8 × 0.8 mm, altogether similar in size to a Millar pressure catheter. The LED catheter had minimal impact on aortic flow and heart rate in Langendorff perfusion and did not impact stability of the left ventricule of the working heart. Changes in transmural absorbance spectra were deconvoluted using a library of chromophore reference spectra to quantify the relative contribution of specific chromophores to the changes in measured absorbance. This broad-band spectral deconvolution approach eliminated errors that may result from simple dual-wavelength absorbance intensity. The myoglobin oxygenation level was only 82.2 ± 3.0%, whereas cytochrome
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Registered trials
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