ArticleMicrobial cell factories2017
Systematic pathway engineering of Corynebacterium glutamicum S9114 for L-ornithine production.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Harnessing prokaryotic amino acid transporters for metabolic engineering: mechanisms and biotechnological applications.Synthetic and systems biotechnology · 2026Review
- Enhanced l-serine synthesis inSynthetic and systems biotechnology · 2025Article
- Comprehensive evaluation of the capacities of microbial cell factories.Nature communications · 2025Article
- Fermentation improves antioxidant capacity and γ-aminobutyric acid content of Ganmai Dazao Decoction by lactic acid bacteria.Frontiers in microbiology · 2023Article
- Improvement in L-ornithine production from mannitol via transcriptome-guided genetic engineering in Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2022Article
- Enhanced L-ornithine production from glucose and sucrose via manipulation of the fructose metabolic pathway in Corynebacterium glutamicum.Bioresources and bioprocessing · 2022Article
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Development of a DNA double-strand break-free base editing tool inMetabolic engineering communications · 2020Article
- Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in L-ornithine production.Microbial cell factories · 2020Article
- Metabolic and evolutionary responses ofBiotechnology for biofuels · 2020Article
- Recent Advances of L-ornithine Biosynthesis in Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2019Review
- EngineeringBiotechnology for biofuels · 2019Article
- Optimization of ʟ-ornithine production in recombinant Corynebacterium glutamicum S9114 by cg3035 overexpression and manipulating the central metabolic pathway.Microbial cell factories · 2018Article
- Improved L-ornithine production in Corynebacterium crenatum by introducing an artificial linear transacetylation pathway.Journal of industrial microbiology & biotechnology · 2018Article
- Pathway engineering in3 Biotech · 2018Article
- Improvement of L-ornithine production by attenuation of argF in engineered Corynebacterium glutamicum S9114.AMB Express · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundL-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer.
resultsIn this study, Corynebacterium glutamicum S9114, a high glutamate-producing strain, was developed for L-ornithine production by pathway engineering. First, argF was deleted to block L-ornithine to citrulline conversion. To improve L-ornithine production, ncgl1221 encoding glutamate transporter, argR encoding arginine repressor, and putP encoding proline transporter were disrupted. This base strain was further engineered by attenuating oxoglutarate dehydrogenase to increase L-ornithine production. Plasmid-based overexpression of argCJBD operon and lysine/arginine transport protein LysE was tested to strengthen L-ornithine synthesis and transportation. This resulted in efficient L-ornithine production at a titer of 18.4 g/L.
conclusionThese results demonstrate the potential of Corynebacterium glutamicum S9114 for efficient L-ornithine production and provide new targets for strain development.
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