ArticlePLoS biology2017
Genome-wide identification of bacterial plant colonization genes.
Article in PLoS biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
115 citing papers in PubMed, 222 citations in OpenAlex.
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- CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization.Nature communications · 2026Article
- Flavonoid-mediated bacterial spermidine biosynthesis enhances vitamin accumulation in tomato fruits.Nature communications · 2026Article
- Unraveling fitness landscapes in plant-associated Bacteroidota.ISME communications · 2026Article
- Construction of a randomly barcoded insertional mutant library in the filamentous fungusbioRxiv : the preprint server for biology · 2025Article
- Bacterial fitness for plant colonization is influenced by plant growth substrate.The New phytologist · 2025Article
- Root-exuded sugars as drivers of rhizosphere microbiome assembly.Plant biotechnology (Tokyo, Japan) · 2025Article
- Breaking the reproducibility barrier with standardized protocols for plant-microbiome research.PLoS biology · 2025Article
- Potential and challenges for application of microbiomes in agriculture.The Plant cell · 2025Review
- Genetic Drivers of Plant Root Colonisation by the Biocontrol Agent Pseudomonas protegens Pf-5.Environmental microbiology reports · 2025Article
- Engineered Membrane Vesicle Production viaACS synthetic biology · 2025Article
- An effluent pump family distributed across plant commensal bacteria conditions host- and organ-specific glucosinolate detoxification.Nature communications · 2025Article
- Context matters: assessing the impacts of genomic background and ecology on microbial biosynthetic gene cluster evolution.mSystems · 2025Review
- Plant miRNAs influence soil bacterial growth and amino acid uptake, restructuring community composition.ISME communications · 2025Article
- Nutrient Requirements Shape the Preferential Habitat of Allorhizobium vitis VAR03-1, a Commensal Bacterium, in the Rhizosphere of Arabidopsis thaliana.Plant & cell physiology · 2024Article
- Community standards and future opportunities for synthetic communities in plant-microbiota research.Nature microbiology · 2024Review
- Optimized CRISPR Interference System for InvestigatingACS synthetic biology · 2024Article
- Factors governing attachment ofmSystems · 2024Article
55 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 1 country.
Funding
Abstract
Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium, to establish a genome-wide map of bacterial genes required for colonization of the Arabidopsis thaliana root system. We identified 115 genes (2% of all P. simiae genes) with functions that are required for maximal competitive colonization of the root system. Among the genes we identified were some with obvious colonization-related roles in motility and carbon metabolism, as well as 44 other genes that had no or vague functional predictions. Independent validation assays of individual genes confirmed colonization functions for 20 of 22 (91%) cases tested. To further characterize genes identified by our screen, we compared the functional contributions of P. simiae genes to growth in 90 distinct in vitro conditions by RB-TnSeq, highlighting specific metabolic functions associated with root colonization genes. Our analysis of bacterial genes by sequence-driven saturation mutagenesis revealed a genome-wide map of the genetic determinants of plant root colonization and offers a starting point for targeted improvement of the colonization capabilities of plant-beneficial microbes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.