Evidence map›Paper›PMID 28937735›Full record

ArticleACS chemical biology2017

Whole-Genome Shotgun Sequencing of Two β-Proteobacterial Species in Search of the Bulgecin Biosynthetic Cluster.

Mark E Horsman, Daniel R Marous, Rongfeng Li, Ryan A Oliver, Byungjin Byun, Scott J Emrich, Bill Boggess, Craig A Townsend, Shahriar Mobashery

Open access · hybridAbstract read
In one paragraph

Article in ACS chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.2field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 32 citations in OpenAlex.

  1. Review
  2. Review
  3. Bacterial peptidoglycan as a living polymer.Current opinion in chemical biology · 2025
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  5. TheiScience · 2024
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  8. Article
  9. Review
  10. Article
  11. Unconventional Antibacterials and Adjuvants.Accounts of chemical research · 2021
    Article
  12. Article
  13. Article
  14. Article
  15. Constructing and deconstructing the bacterial cell wall.Protein science : a publication of the Protein Society · 2020
    Review
  16. Crossroads of Antibiotic Resistance and Biosynthesis.Journal of molecular biology · 2019
    Review
  17. Journal of natural products · 2019
    Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Rongfeng LiDepartment of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
Ryan A OliverDepartment of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
Byungjin Byun
Scott J Emrich
Bill Boggess
Craig A TownsendDepartment of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
Shahriar MobasheryORCID 0000-0002-7695-7883
Johns Hopkins University · US

Funding

Biosynthesis of Beta-Lactam AntibioticsR37AI014937 · NIAID · JOHNS HOPKINS UNIVERSITY · PI TOWNSEND, CRAIG ARTHUR · 2004 to 2013
$5.2M
Penicillin-Binding Proteins, Mechanism and InhibitionR01GM061629 · NIGMS · WAYNE STATE UNIVERSITY · PI MOBASHERY, SHAHRIAR · 2001 to 2018
$5.1M
Biosynthesis of Beta Lactam AntibioticsR01AI121072 · NIAID · JOHNS HOPKINS UNIVERSITY · PI TOWNSEND, CRAIG ARTHUR · 2016 to 2025
$4.9M
NIAID NIH HHS R01 AI121072NIAID NIH HHS R37 AI014937NIGMS NIH HHS R01 GM061629
6 · The paper itself

Abstract

We have produced draft whole-genome sequences for two bacterial strains reported to produce the bulgecins as well as NRPS-derived monobactam β-lactam antibiotics. We propose classification of ATCC 31363 as Paraburkholderia acidophila. We further reaffirm that ATCC 31433 (Burkholderia ubonensis subsp. mesacidophila) is a taxonomically distinct producer of bulgecins with notable gene regions shared with Paraburkholderia acidophila. We use RAST multiple-gene comparison and MASH distancing with published genomes to order the draft contigs and identify unique gene regions for characterization. Forty-eight natural-product gene clusters are presented from PATRIC (RASTtk) and antiSMASH annotations. We present evidence that the 10 genes that follow the sulfazecin and isosulfazecin pathways in both species are likely involved in bulgecin A biosynthesis.

Indexed as

Genome, BacterialBurkholderiaceaeGene Expression Regulation, BacterialGlycopeptidesMultigene FamilybulgecinGlycopeptides

Identifiers

PMID28937735
PMCPMC5653948
OpenAlexW2758040265

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.