ArticleNature chemistry2017
A reductive aminase from Aspergillus oryzae.
Article in Nature chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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101 citing papers in PubMed, 382 citations in OpenAlex.
- An Engineered Enzyme Catalyzing Tandem Reductive Aminations for Synthesizing Tertiary Amines.Angewandte Chemie (International ed. in English) · 2026Article
- Substrate-Guided Engineering of Imine Reductases: A Strategy for Synthesizing Tertiary Amine N-Heterocycles.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Data-driven construction of an imine reductase library capable of broad-scope reductive amination at equimolar substrate concentrations.Nature communications · 2026Article
- Biocatalytic Radical C(spResearch square · 2026Article
- Evolution and Impact of Imine Reductases (IREDs) Research: A Knowledge Mapping Approach.Molecular biotechnology · 2026Review
- Stereoconvergent Photo-Biocatalytic Cascade to Optically Enriched Amines from Racemic Carboxylic Acids.ACS omega · 2026Article
- Nitroreductase-triggered indazole formation.Nature communications · 2026Article
- Enantioselective Gram-Scale Synthesis of Rasagiline via Chiral Phosphoric Acid-Catalyzed Reduction of Cyclic Imine.Chemistry, an Asian journal · 2026Article
- Engineered C-N Lyases for Stereoselective Synthesis of Tertiary Amines.Angewandte Chemie (International ed. in English) · 2025Article
- Nucleophilic α-Functionalization of Benzyl Amines Using an Engineered Threonine Aldolase.Journal of the American Chemical Society · 2025Article
- High-ThroughputACS central science · 2025Article
- Stereoselective Biosynthesis of Bichiral Amino Alcohols from Diketone via Difunctionalization.ACS omega · 2025Article
- Phase-Separated Multienzyme Condensates for Efficient Synthesis of Imines from Carboxylic Acids with Enhanced Dual-Cofactor Recycling.International journal of molecular sciences · 2025Article
- Advances in the Molecular Modification of Microbial ω-Transaminases for Asymmetric Synthesis of Bulky Chiral Amines.Microorganisms · 2025Review
- Synthesis of Substituted Acyclic and Cyclic N-Alkylhydrazines by Enzymatic Reductive Hydrazinations.Chembiochem : a European journal of chemical biology · 2025Article
- Direct carbonyl reductive functionalizations by diphenylphosphine oxide.Science advances · 2025Article
- Discovery and Synthetic Applications of a NAD(P)H-Dependent Reductive Aminase fromACS catalysis · 2025Article
- In situ characterization of amine-forming enzymes shows altered oligomeric state.Protein science : a publication of the Protein Society · 2025Article
- Expanding the repertoire of imine reductases by mining divergent biosynthetic pathways for promiscuous reactivity.Chem catalysis · 2024Article
- Asymmetric Synthesis of 2-Arylethylamines: A Metal-Free Review of the New Millennium.Molecules (Basel, Switzerland) · 2024Review
41 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reductive amination is one of the most important methods for the synthesis of chiral amines. Here we report the discovery of an NADP(H)-dependent reductive aminase from Aspergillus oryzae (AspRedAm, Uniprot code Q2TW47) that can catalyse the reductive coupling of a broad set of carbonyl compounds with a variety of primary and secondary amines with up to >98% conversion and with up to >98% enantiomeric excess. In cases where both carbonyl and amine show high reactivity, it is possible to employ a 1:1 ratio of the substrates, forming amine products with up to 94% conversion. Steady-state kinetic studies establish that the enzyme is capable of catalysing imine formation as well as reduction. Crystal structures of AspRedAm in complex with NADP(H) and also with both NADP(H) and the pharmaceutical ingredient (R)-rasagiline are reported. We also demonstrate preparative scale reductive aminations with wild-type and Q240A variant biocatalysts displaying total turnover numbers of up to 32,000 and space time yields up to 3.73 g l
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