ArticleModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc2018
In situ protein detection with enhanced specificity using DNA-conjugated antibodies and proximity ligation.
Article in Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- The influences of ApoE isoforms on endothelial adherens junctions and actin cytoskeleton responding to mCRP.Angiogenesis · 2024Article
- Therapeutic Oligonucleotides: An Outlook on Chemical Strategies to Improve Endosomal Trafficking.Cells · 2023Review
- Peripheral apolipoprotein E proteins and their binding to LRP1 antagonize Alzheimer's disease pathogenesis in the brain during peripheral chronic inflammation.Neurobiology of aging · 2023Article
- Specific Detection of Physiological S129 Phosphorylated α-Synuclein in Tissue Using Proximity Ligation Assay.Journal of Parkinson's disease · 2023Article
- Sensitive Protein Detection Using Site-Specifically Oligonucleotide-Conjugated Nanobodies.Analytical chemistry · 2022Article
- Article
- Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes.RSC chemical biology · 2021Article
- Proximity Ligation Assay as a Tool for Antibody Validation in Human Tissues.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- If this is true, what does it imply? How end-user antibody validation facilitates insights into biology and disease.Asian journal of urology · 2019Review
- Smartphone-based clinical diagnostics: towards democratization of evidence-based health care.Journal of internal medicine · 2019Review
- Improved relapse-free survival on aromatase inhibitors in breast cancer is associated with interaction between oestrogen receptor-α and progesterone receptor-b.British journal of cancer · 2018Article
- Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes.Scientific reports · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibodies are important tools in anatomical pathology and research, but the quality of in situ protein detection by immunohistochemistry greatly depends on the choice of antibodies and the abundance of the targeted proteins. Many antibodies used in scientific research do not meet requirements for specificity and sensitivity. Accordingly, methods that improve antibody performance and produce quantitative data can greatly advance both scientific investigations and clinical diagnostics based on protein expression and in situ localization. We demonstrate here protocols for antibody labeling that allow specific protein detection in tissues via bright-field in situ proximity ligation assays, where each protein molecule must be recognized by two antibodies. We further demonstrate that single polyclonal antibodies or purified serum preparations can be used for these dual recognition assays. The requirement for protein recognition by pairs of antibody conjugates can significantly improve specificity of protein detection over single-binder assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.