Evidence map›Paper›PMID 28936798›Full record

ArticleMolecular neurobiology2018

Conditioned Medium of Human Adipose Mesenchymal Stem Cells Increases Wound Closure and Protects Human Astrocytes Following Scratch Assay In Vitro.

Eliana Baez-Jurado, Oscar Hidalgo-Lanussa, Gina Guio-Vega, Ghulam Md Ashraf, Valentina Echeverria, Gjumrakch Aliev, George E Barreto

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

  1. Pooled it
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  5. Current advances in in vitro models of central nervous system trauma.Current opinion in biomedical engineering · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 5 countries.

Eliana Baez-JuradoDepartamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia.
Oscar Hidalgo-LanussaDepartamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia.
Gina Guio-VegaDepartamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia.
Ghulam Md AshrafKing Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Valentina EcheverriaResearch & Development Service, Bay Pines VA Healthcare System, Bay Pines, FL, 33744, USA.
Gjumrakch AlievInstitute of Physiologically Active Compounds, Russian Academy of Sciences, Chernogolovka, Moscow Region, 142432, Russia.
George E BarretoDepartamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia. gsampaio@javeriana.edu.co.ORCID http://orcid.org/0000-0002-6644-1971
Pontificia Universidad Javeriana · COInternational Biomedical (United States) · USKing Abdulaziz University · SASan Sebastián University · CL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Astrocytes perform essential functions in the preservation of neural tissue. For this reason, these cells can respond with changes in gene expression, hypertrophy, and proliferation upon a traumatic brain injury event (TBI). Different therapeutic strategies may be focused on preserving astrocyte functions and favor a non-generalized and non-sustained protective response over time post-injury. A recent strategy has been the use of the conditioned medium of human adipose mesenchymal stem cells (CM-hMSCA) as a therapeutic strategy for the treatment of various neuropathologies. However, although there is a lot of information about its effect on neuronal protection, studies on astrocytes are scarce and its specific action in glial cells is not well explored. In the present study, the effects of CM-hMSCA on human astrocytes subjected to scratch assay were assessed. Our findings indicated that CM-hMSCA improved cell viability, reduced nuclear fragmentation, and preserved mitochondrial membrane potential. These effects were accompanied by morphological changes and an increased polarity index thus reflecting the ability of astrocytes to migrate to the wound stimulated by CM-hMSCA. In conclusion, CM-hMSCA may be considered as a promising therapeutic strategy for the protection of astrocyte function in brain pathologies.

Indexed as

Biological AssayAdipose TissueAntioxidantsAstrocytesCell MovementCell ShapeCell SurvivalCulture Media, ConditionedDNA FragmentationHumansMembrane Potential, MitochondrialMesenchymal Stem CellsModels, BiologicalNeuroprotectionWound HealingAntioxidantsCulture Media, ConditionedBrain injuryConditioned mediumHuman astrocytesMesenchymal stem cellsMigrationScratch

Identifiers

PMID28936798
OpenAlexW2759629497

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.