Evidence map›Paper›PMID 28936471›Full record

ArticleCellular and molecular gastroenterology and hepatology2017

Analysis of Hepatitis C Virus Particle Heterogeneity in Immunodeficient Human Liver Chimeric

Ursula Andreo, Ype P de Jong, Margaret A Scull, Jing W Xiao, Koen Vercauteren, Corrine Quirk, Michiel C Mommersteeg, Sonia Bergaya, Arjun Menon, Edward A Fisher and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular gastroenterology and hepatology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Mice Engrafted with Human Liver Cells.Seminars in liver disease · 2024
    Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Heterogeneity of Hepatitis C Virus Particles and Their Evolution During Infection.Cellular and molecular gastroenterology and hepatology · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Ursula AndreoCenter for the Study of Hepatitis C, The Rockefeller University, New York, New York.
Ype P de JongCenter for the Study of Hepatitis C, The Rockefeller University, New York, New York.
Margaret A ScullCenter for the Study of Hepatitis C, The Rockefeller University, New York, New York.
Jing W XiaoCenter for the Study of Hepatitis C, The Rockefeller University, New York, New York.
Koen VercauterenCenter for the Study of Hepatitis C, The Rockefeller University, New York, New York.
Corrine QuirkCenter for the Study of Hepatitis C, The Rockefeller University, New York, New York.
Michiel C MommersteegCenter for the Study of Hepatitis C, The Rockefeller University, New York, New York.
Sonia BergayaDivision of Cardiology, Department of Medicine, New York University Langone Medical Center, New York, New York.
Arjun MenonDivision of Cardiology, Department of Medicine, New York University Langone Medical Center, New York, New York.
Edward A FisherDivision of Cardiology, Department of Medicine, New York University Langone Medical Center, New York, New York.
Charles M RiceCenter for the Study of Hepatitis C, The Rockefeller University, New York, New York.
Rockefeller University · USNYU Langone Health · USCornell University · US

Funding

Translational Research CoreP30DK020541 · NIDDK · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI JEFFREY E. PESSIN · 2015 to 2026
$27.7M
Modeling human hepatotropic infections in complex tissue organoidsR01DK085713 · NIDDK · ROCKEFELLER UNIVERSITY · PI BHATIA, SANGEETA N., RICE, CHARLES M · 2009 to 2013
$5.6M
The role of innate immune evasion in hepatitis C virus infectionK08DK090576 · NIDDK · WEILL MEDICAL COLL OF CORNELL UNIV · PI DE JONG, YPE PETER · 2011 to 2015
$760k
Characterization and Consequences of HCV Interaction with Host LipoproteinsF32AI091207 · NIAID · ROCKEFELLER UNIVERSITY · PI SCULL, MARGARET ADELE · 2010 to 2012
$146k
NIAID NIH HHS F32 AI091207NIDDK NIH HHS K08 DK090576NIDDK NIH HHS P30 DK020541NIDDK NIH HHS R01 DK085713
6 · The paper itself

Abstract

BACKGROUND &

aimsHepatitis C virus (HCV) is a leading cause of chronic liver diseases and the most common indication for liver transplantation in the United States. HCV particles in the blood of infected patients are characterized by heterogeneous buoyant densities, likely owing to HCV association with lipoproteins. However, clinical isolates are not infectious in vitro and the relative infectivity of the particles with respect to their buoyant density therefore cannot be determined, pointing to the need for better in vivo model systems.

methodsTo analyze the evolution of the buoyant density of in vivo-derived infectious HCV particles over time, we infected immunodeficient human liver chimeric fumaryl acetoacetate hydrolase

resultsSimilar to the severe combined immunodeficiency disease/Albumin-urokinase plasminogen activator human liver chimeric mouse model, density fractionation of infectious mouse serum showed higher infectivity in the low-density fractions early after infection. However, over the course of the infection, viral particle heterogeneity increased and the overall in vitro infectivity diminished without loss of the human liver graft over time. In mice provided with a sucrose-rich diet we observed a minor shift in HCV infectivity toward lower density that correlated with a redistribution of triglycerides and cholesterol among lipoproteins.

conclusionsOur work indicates that the heterogeneity in buoyant density of infectious HCV particles evolves over the course of infection and can be influenced by diet.

Indexed as

Alb-uPA, Albumin-urokinase plasminogen activatorapo, apolipoproteinCETP, cholesterol ester transfer proteinFAH, fumaryl acetoacetate hydrolaseFNRG, absence of fumaryl acetoacetate hydrolase on a immunodeficient NOD Rag gamma IL2 deficient mouse backgroundFPLC, fast-performance liquid chromatographyHCVHCVcc, cell culture–derived hepatitis C virusHCV, hepatitis C virusHDL, high-density lipoproteinHuman Liver Chimeric MiceLipoproteinLVP, lipoviroparticleMouse ModelNRG, nod rag γNTBC, nitisinonePBS, phosphate-buffered salineSCID, severe combined immunodeficiency diseaseVLDL, very low density lipoprotein

Identifiers

PMID28936471
PMCPMC5602752
OpenAlexW2737963197

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.