ArticleAdvanced optical materials2017
Programmable Microfluidic Synthesis of Over One Thousand Uniquely Identifiable Spectral Codes.
Article in Advanced optical materials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- Decoupling Fabrication From Encoding: DNA-Addressable Template Microparticles for Large, User-Defined Optical Barcode Libraries.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Lab on a Particle Technologies.Analytical chemistry · 2024Review
- Phosphatase specificity principles uncovered by MRBLE:Dephos and global substrate identification.Molecular systems biology · 2023Article
- Rapid parallel generation of a fluorescently barcoded drop library from a microtiter plate using the plate-interfacing parallel encapsulation (PIPE) chip.Lab on a chip · 2022Article
- A bead-based method for high-throughput mapping of the sequence- and force-dependence of T cell activation.Nature methods · 2022Article
- Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles.ACS nano · 2022Review
- MRBLE-pep Measurements Reveal Accurate Binding Affinities for B56, a PP2A Regulatory Subunit.ACS measurement science au · 2021Article
- μCB-seq: microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells.Lab on a chip · 2020Article
- μDamID: A Microfluidic Approach for Joint Imaging and Sequencing of Protein-DNA Interactions in Single Cells.Cell systems · 2020Article
- Lanthanide-Based Optical Probes of Biological Systems.Cell chemical biology · 2020Review
- Article
- High-throughput screening by droplet microfluidics: perspective into key challenges and future prospects.Lab on a chip · 2020Article
- Tetracationic Bis-Triarylborane 1,3-Butadiyne as a Combined Fluorimetric and Raman Probe for Simultaneous and Selective Sensing of Various DNA, RNA, and Proteins.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- micrIO: an open-source autosampler and fraction collector for automated microfluidic input-output.Lab on a chip · 2020Article
- MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically encoded hydrogel beads using a simple single-layer microfluidic device.Microsystems & nanoengineering · 2020Article
- Ultrasimple Single-Cell Detection of Multiple Cytokines by a Nanowell Chip Integrated with Encoded Microarrays.ACS sensors · 2019Article
- Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads.eLife · 2019Article
- An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs).PloS one · 2019Article
- An intrinsically disordered proteins community for ELIXIR.F1000Research · 2019Article
- Ensemble multicolour FRET model enables barcoding at extreme FRET levels.Nature nanotechnology · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Encoded microparticles have become a powerful tool for a wide array of applications, including high-throughput sample tracking and massively parallel biological multiplexing. Spectral encoding, where particles are encoded with distinct luminescence spectra, provides a particularly appealing encoding strategy because of the ease of reading codes and assay flexibility. To date, spectral encoding has been limited in the number of codes that can be accurately resolved. Here, we demonstrate an automated 5-dimensional spectral encoding scheme using lanthanide nanophosphors that is capable of producing isotropic spherical microparticles with up to 1,100 unique codes, which we term MRBLEs (Microspheres with Ratiometric Barcode Lanthanide Encoding). We further develop a quantitative framework for evaluating global ability to distinguish codes and demonstrate that for six different sets of MRBLEs ranging from 106 to 1,101 codes in size, > 98% of MRBLEs can be assigned to a code with 99.99% confidence. These > 1,000 code sets represent the largest spectral code libraries built to date. We expect that these MRBLEs will enable a wide variety of novel multiplexed assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.