ArticleRSC advances2017
Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethyl sulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability.
Article in RSC advances, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 65 citations in OpenAlex.
- Advanced sperm preservation techniques in yellow spotted mountain newts Neurergus derjugini enhance genetic management and conservation efforts.Scientific reports · 2025Article
- Physicochemical Mechanisms of Protection Offered by Agarose Encapsulation during Cryopreservation of Mammalian Cells in the Absence of Membrane-Penetrating Cryoprotectants.ACS applied bio materials · 2023Article
- A highly active mineral-based ice nucleating agent supportsJournal of the Royal Society, Interface · 2023Article
- Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival.Biomolecules · 2022Article
- Toxicity profiles and protective effects of antifreeze proteins from insect in mammalian models.Toxicology letters · 2022Article
- Intracellular and Extracellular Antifreeze Protein Significantly Improves Mammalian Cell Cryopreservation.Biomolecules · 2022Article
- An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos.The Journal of experimental biology · 2022Article
- Red Blood Cell Cryopreservation with Minimal Post-Thaw Lysis Enabled by a Synergistic Combination of a Cryoprotecting Polyampholyte with DMSO/Trehalose.Biomacromolecules · 2022Article
- Human serum albumin and chromatin condensation rescue ex vivo expanded γδ T cells from the effects of cryopreservation.Cryobiology · 2021Article
- Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation.International journal of molecular sciences · 2021Article
- DeepFoci: Deep learning-based algorithm for fast automatic analysis of DNA double-strand break ionizing radiation-induced foci.Computational and structural biotechnology journal · 2021Article
- Simple Syntheses of New Pegylated Trehalose Derivatives as a Chemical Tool for Potential Evaluation of Cryoprotectant Effects on Cell Membrane.Molecules (Basel, Switzerland) · 2020Article
- A beetle antifreeze protein protects lactate dehydrogenase under freeze-thawing.International journal of biological macromolecules · 2019Article
- Challenges and Contradictions of Metal Nano-Particle Applications for Radio-Sensitivity Enhancement in Cancer Therapy.International journal of molecular sciences · 2019Article
- Methods of Cryoprotectant Preservation: Allogeneic Cellular Bone Grafts and Potential Effects.BioMed research international · 2019Review
- Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants.Scientific reports · 2018Article
- AFM Monitoring the Influence of Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties.Frontiers in physiology · 2018Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 3 countries.
Funding
Abstract
In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab-initio modelling (molecular dynamics, DFT), Raman spectroscopy, Differential Scanning Calorimetry and X-Ray Diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.