ArticleCellular and molecular bioengineering2017
Microfibrous Scaffolds Enhance Endothelial Differentiation and Organization of Induced Pluripotent Stem Cells.
Article in Cellular and molecular bioengineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Technology for the formation of engineered microvascular network models and their biomedical applications.Nano convergence · 2024Review
- Small diameter vascular grafts: progress on electrospinning matrix/stem cell blending approach.Frontiers in bioengineering and biotechnology · 2024Review
- Engineering Spatiotemporal Control in Vascularized Tissues.Bioengineering (Basel, Switzerland) · 2022Review
- Recent trends in bioartificial muscle engineering and their applications in cultured meat, biorobotic systems and biohybrid implants.Communications biology · 2022Review
- Article
- Micro- and nanoscale biophysical cues for cardiovascular disease therapy.Nanomedicine : nanotechnology, biology, and medicine · 2021Review
- Engineering Cardiovascular Tissue Chips for Disease Modeling and Drug Screening Applications.Frontiers in bioengineering and biotechnology · 2021Review
- Fiber Scaffold Patterning for Mending Hearts: 3D Organization Bringing the Next Step.Advanced healthcare materials · 2020Review
- Vascularization in tissue engineering: fundamentals and state-of-art.Progress in biomedical engineering (Bristol, England) · 2020Article
- Bioceramic akermanite enhanced vascularization and osteogenic differentiation of human induced pluripotent stem cells in 3D scaffoldsRSC advances · 2019Article
- iPSCs: A powerful tool for skeletal muscle tissue engineering.Journal of cellular and molecular medicine · 2019Review
- Biomaterials and Advanced Biofabrication Techniques in hiPSCs Based Neuromyopathic Disease Modeling.Frontiers in bioengineering and biotechnology · 2019Review
- Vascularization of Engineered Spatially Patterned Myocardial Tissue Derived From Human Pluripotent Stem CellsFrontiers in bioengineering and biotechnology · 2019Article
- Treatment of volumetric muscle loss in mice using nanofibrillar scaffolds enhances vascular organization and integration.Communications biology · 2019Article
- Environmental Specification of Pluripotent Stem Cell Derived Endothelial Cells Toward Arterial and Venous Subtypes.Frontiers in bioengineering and biotechnology · 2019Review
- Nanopatterned bioresorbable elastomeric scaffolds to promote neural, glial, and endothelial differentiation using human embryonic and induced pluripotent stem cells.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
introductionHuman induced pluripotent stem cells (iPSCs) are a promising source of endothelial cells (iPSC-ECs) for engineering three-dimensional (3D) vascularized cardiac tissues. To mimic cardiac microvasculature, in which capillaries are oriented in parallel, we hypothesized that endothelial differentiation of iPSCs within topographically aligned 3D scaffolds would be a facile one-step approach to generate iPSC-ECs as well as induce aligned vascular organization.
methodsHuman iPSCs underwent endothelial differentiation within electrospun 3D polycaprolactone (PCL) scaffolds having either randomly oriented or parallel-aligned microfibers. Using transcriptional, protein, and endothelial functional assays, endothelial differentiation was compared between conventional two-dimensional (2D) films and 3D scaffolds having either randomly oriented or aligned microfibers. Furthermore, the role of parallel-aligned microfiber patterning on the organization of vessel-like networks was assessed.
resultsThe cells in both the randomly oriented and aligned 3D scaffolds demonstrated an 11-fold upregulation in gene expression of the endothelial phenotypic marker, CD31, compared to cells on 2D films. This upregulation corresponded to >3-fold increase in CD31 protein expression in 3D scaffolds, compared to 2D films. Concomitantly, other endothelial phenotypic markers including CD144 and endothelial nitric oxide synthase also showed significant transcriptional upregulation in 3D scaffolds by >7-fold, compared to 2D films. Nitric oxide production, which is characteristic of endothelial function, was produced 4-fold more abundantly in 3D scaffolds, compared to on 2D PCL films. Within aligned scaffolds, the iPSC-ECs displayed parallel-aligned vascular-like networks with 70% longer branch length, compared to cells in randomly oriented scaffolds, suggesting that fiber topography modulates vascular network-like formation and patterning.
conclusionTogether, these results demonstrate that 3D scaffold structure promotes endothelial differentiation, compared to 2D substrates, and that aligned topographical patterning induces anisotropic vascular network organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.