ArticleScientific reports2017
Antibacterial and immunomodulatory activities of insect defensins-DLP2 and DLP4 against multidrug-resistant Staphylococcus aureus.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58 citing papers in PubMed, 108 citations in OpenAlex.
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- Potential health benefits of insect bioactive metabolites and consumer attitudes towards edible insects.NPJ science of food · 2025Review
- Impact ofInsects · 2025Article
- The antimicrobial peptide Cec4 has therapeutic potential against clinical carbapenem-resistantMicrobiology spectrum · 2025Article
- Novel Strategies for Preventing Fungal Infections-Outline.Pathogens (Basel, Switzerland) · 2025Review
- The Black Soldier FlyCurrent research in microbial sciences · 2025Article
- Insect Brain Proteomics: A Case Study of Periplaneta americana.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms.AMB Express · 2024Article
- Immunomodulatory Effects of the Tobacco Defensin NaD1.Antibiotics (Basel, Switzerland) · 2024Article
- Article
- Therapeutic Potential of Insect Defensin DLP4 AgainstPharmaceutics · 2024Article
- Principle Investigation and Method Standardization of Inhibition Zone Assay Based on Antimicrobial Peptides Extracted from Black Soldier Fly Larvae.Biotech (Basel (Switzerland)) · 2024Article
- Black soldier fly (Health science reports · 2024Review
- A novel family of defensin-like peptides from Hermetia illucens with antibacterial properties.BMC microbiology · 2024Article
- An attacin antimicrobial peptide, Hill_BB_C10074, from Hermetia illucens with anti-Pseudomonas aeruginosa activity.BMC microbiology · 2023Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA), are the most frequent cause of sepsis, which urgently demanding new drugs for treating infection. Two homologous insect CSαβ peptides-DLP2 and DLP4 from Hermetia illucens were firstly expressed in Pichia pastoris, with the yields of 873.5 and 801.3 mg/l, respectively. DLP2 and DLP4 displayed potent antimicrobial activity against Gram-positive bacteria especially MRSA and had greater potency, faster killing, and a longer postantibiotic effect than vancomycin. A 30-d serial passage of MRSA in the presence of DLP2/DLP4 failed to produce resistant mutants. Macromolecular synthesis showed that DLP2/DLP4 inhibited multi-macromolecular synthesis especially for RNA. Flow cytometry and electron microscopy results showed that the cell cycle was arrested at R-phase; the cytoplasmic membrane and cell wall were broken by DLP2/DLP4; mesosome-like structures were observed in MRSA. At the doses of 3‒7.5 mg/kg DLP2 or DLP4, the survival of mice challenged with MRSA were 80‒100%. DLP2 and DLP4 reduced the bacterial translocation burden over 95% in spleen and kidneys; reduced serum pro-inflammatory cytokines levels; promoted anti-inflammatory cytokines levels; and ameliorated lung and spleen injury. These data suggest that DLP2 and DLP4 may be excellent candidates for novel antimicrobial peptides against staphylococcal infections.
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